2.1.1.159: theobromine synthase

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For detailed information about theobromine synthase, go to the full flat file.

Reaction

Synonyms

3,7-dimethylxanthine methyltransferase, 3-N-methyltransferase, 3NMT, 7-methylxanthine 3-N-methyltransferase, 7-methylxanthine methyltransferase, 7-methylxanthine N-methyltransferase, caffeine synthase, caffeine synthase 1, CCS1, coffee caffeine synthase 1, CS, CtCS6, CTS, CTS1, CTS2, DXMT, DXMT1, MXMT, MXMT1, MXMT2, N-3 methyltransferase, N-methyltransferase, TCS1, theobromine synthase 2

ECTree

     2 Transferases

         2.1 Transferring one-carbon groups

             2.1.1 Methyltransferases

                2.1.1.159 theobromine synthase

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Results	in table
1	Activating Compound
26	AA Sequence
5	Application
1	CAS Registry Number
15	Cloned(Commentary)
1	Cofactor
2	Crystallization (Commentary)
4	Expression
1	General Information
7	Inhibitors
18	KM Value [mM]
4	Localization
3	Metals/Ions
15	Molecular Weight [Da]
36	Natural Substrates/ Products (Substrates)
33	Organism
6	Pathway
8	pH Optimum
3	pH Range
2	pI Value
4	Protein Variants
6	Purification (Commentary)
3	Reaction
33	Reference
41	Source Tissue
9	Specific Activity [micromol/min/mg]
76	Substrates and Products (Substrate)
14	Subunits
36	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.1.1.159 - theobromine synthase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 1 mM S-adenosyl-L-cysteine, and 1 mM theobromine, 1-3 days, 20°C, X-ray diffraction structure determination and analysis at 2.5-2.7 A resolution

Coffea canephora

A4GE70

667101

purified recombinant wild-type and selenomethionine-labeled DXMT, 23-28% PEG 3350, 0.2 M LiCl, 0.1 M Tris-HCl, pH 8.5-8.7, 2 mM DTT, 2 mM S-adenosyl-L-cysteine, and 2 mM theobromine, 1-3 days, 20°C, plate-like crystals, X-ray diffraction structure determination and analysis at 2.0-2.7 A resolution, molecular replacement

Coffea canephora

-

670598