Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.1.159: theobromine synthase

This is an abbreviated version!
For detailed information about theobromine synthase, go to the full flat file.

Word Map on EC 2.1.1.159

Reaction

S-adenosyl-L-methionine
+
7-methylxanthine
=
S-adenosyl-L-homocysteine
+
3,7-Dimethylxanthine
+
H+

Synonyms

3,7-dimethylxanthine methyltransferase, 3-N-methyltransferase, 3NMT, 7-methylxanthine 3-N-methyltransferase, 7-methylxanthine methyltransferase, 7-methylxanthine N-methyltransferase, caffeine synthase, caffeine synthase 1, CCS1, coffee caffeine synthase 1, CS, CtCS6, CTS, CTS1, CTS2, DXMT, DXMT1, MXMT, MXMT1, MXMT2, N-3 methyltransferase, N-methyltransferase, TCS1, theobromine synthase 2

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.159 theobromine synthase

Purification

Purification on EC 2.1.1.159 - theobromine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme 39fold from young leaves by ammonium sulfate fractionation, 3 steps of ion exchange chromatography, and gel filtration
-
native enzyme 523fold from young, developing leaves by ammonium sulfate fractionation, hydroxylapatite, anion exchange, and adenosine affinity chromatography, and gel filtration to homogeneity
-
partially purified by Ni-NTA agarose column chromatography
-
recombinant GST-fusion enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography
recombinant His-tagged DXMT1 from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography, cleavage of the His-tag with tobacco etch virus, TEV, protease, followed by gel filtration