Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.1.159: theobromine synthase

This is an abbreviated version!
For detailed information about theobromine synthase, go to the full flat file.

Word Map on EC 2.1.1.159

Reaction

S-adenosyl-L-methionine
+
7-methylxanthine
=
S-adenosyl-L-homocysteine
+
3,7-Dimethylxanthine
+
H+

Synonyms

3,7-dimethylxanthine methyltransferase, 3-N-methyltransferase, 3NMT, 7-methylxanthine 3-N-methyltransferase, 7-methylxanthine methyltransferase, 7-methylxanthine N-methyltransferase, caffeine synthase, caffeine synthase 1, CCS1, coffee caffeine synthase 1, CS, CtCS6, CTS, CTS1, CTS2, DXMT, DXMT1, MXMT, MXMT1, MXMT2, N-3 methyltransferase, N-methyltransferase, TCS1, theobromine synthase 2

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.159 theobromine synthase

Cloned

Cloned on EC 2.1.1.159 - theobromine synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of MXMT vectors for Agrobacterium tumefaciens-mediated transformation
-
DNA and amino acid sequence determination and analysis, cloning of MXMT vectors for Agrobacterium tumefaciens-mediated transformation
DNA and amino acid sequence determination and analysis, expression in Escherichia coli as wild-type and as Trx-fusion protein
expressed in Escherichia coli BL21-pRil cells
-
expressed in Escherichia coli Rosetta BL21 (DE3) and Saccharomyces cerevisiae INVSC1 cells
expression in Escherichia coli
-
expression of His-tagged DXMT1 in Escherichia coli strain BL21(DE3)
expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), sequence comparisonof caffeine synthetic enzymes from coffee
Coffea sp.
-
gene CaMTL2, cDNA library construction, DNA and amino acid sequence determination and analysis
gene CaMXMT, cDNA library construction, DNA and amino acid sequence determination and analysis, sequence comparison with other species, expression of CaMXMT as GST-fusion enzyme in Escherichia coli strain JM109
gene MXMT2, DNA and amino acid sequence determination and analysis
gene TCS1, cDNA and amino acid sequence determination and analysis, expression in Escherichia coli
isozyme MXMT1, DNA and amnio acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3)
isozyme MXMT1, functional expression in transgenic Nicotiana tabacum plant leaves
isozyme MXMT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of the GST-tagged isozymes in Escherichia coli Bl21(DE3)