Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.1.1.140: (S)-coclaurine-N-methyltransferase

This is an abbreviated version!
For detailed information about (S)-coclaurine-N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.140

Reaction

S-adenosyl-L-methionine
+
(S)-Coclaurine
=
S-adenosyl-L-homocysteine
 +
(S)-N-methylcoclaurine

Synonyms

(S)-Coclaurine N-methyltransferase, 6OMT, CjCNMT, CMT, CNMT, CNMT protein, coclaurine N-methyltransferase, coclaurine-N-methyltransferase, FspC, S-adenosyl-L-methionine:coclaurine N-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.140 (S)-coclaurine-N-methyltransferase

Engineering

Engineering on EC 2.1.1.140 - (S)-coclaurine-N-methyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E204A
4-6% of wild-type activity
E204G
mutation to the corresponding residue of Papaver somniferum reticuline N-methyltransferase. Mutant shows a weak activity with (S)-coclaurine and a more substantial activity with (S)-N-methylcoclaurine. Compared to wild-type, activity with (S)-reticuline is 35fold improved
E207A
60-75% of wild-type activity
F332A
0.6% of wild-typ activity with substrate coclaurine
G331A
negligible effect on activity
H208A
1% of wild-type activity with substrate heliamine, 4% with substrate coclaurine, respectively
R330A
negligible effect on activity
W329A
4% of wild-type activity with substrate heliamine, 0.5% with substrate coclaurine, respectively
Y328A
5% of wild-type activity with substrate heliamine, 10% with substrate coclaurine, respectively
additional information
-
virus-induced gene silencing in opium poppy to functionally characterize six genes potentially involved in papaverine biosynthesis, e.g. the gene encoding coclaurine N-methyltransferase, altered alkaloid content phenotypes, overview. Norlaudanine is only detected in CNMT-suppressed plants