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2.1.1.140: (S)-coclaurine-N-methyltransferase

This is an abbreviated version!
For detailed information about (S)-coclaurine-N-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.140

Reaction

S-adenosyl-L-methionine
+
(S)-Coclaurine
=
S-adenosyl-L-homocysteine
 +
(S)-N-methylcoclaurine

Synonyms

(S)-Coclaurine N-methyltransferase, 6OMT, CjCNMT, CMT, CNMT, CNMT protein, coclaurine N-methyltransferase, coclaurine-N-methyltransferase, FspC, S-adenosyl-L-methionine:coclaurine N-methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.140 (S)-coclaurine-N-methyltransferase

Application

Application on EC 2.1.1.140 - (S)-coclaurine-N-methyltransferase

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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
-
CNMT should be quite useful for biotransformation of the intermediates of alkaloid biosynthesis to N-methyltransferred products
medicine
a Saccharomyces cerevisiae strain is engineered to express seven heterologous enzymes (Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR)), resulting in protoberberine alkaloid production from a simple benzylisoquinoline alkaloid precursor. A number of strategies are implemented to improve flux through the pathway, including enzyme variant screening, genetic copy number variation, and culture optimization. This leads to an over 70-fold increase in canadine titer up to 1.8 mg/l. Increased canadine titers enable extension of the pathway to produce berberine, a major constituent of several traditional medicines in a microbial host. This strain is viable at pilot scale