2.1.1.140: (S)-coclaurine-N-methyltransferase
This is an abbreviated version!
For detailed information about (S)-coclaurine-N-methyltransferase, go to the full flat file.
Word Map on EC 2.1.1.140
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2.1.1.140
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benzylisoquinoline
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n-methylated
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japonica
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coptis
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isoquinoline
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norreticuline
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s-reticuline
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n-methyltransferases
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norcoclaurine
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poppy
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scoulerine
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somniferum
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3\'-hydroxylase
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4\'omt
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magnoflorine
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6-o-methyltransferase
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opium
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papaver
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tetrahydroisoquinoline
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aporphine
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medicine
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biotechnology
- 2.1.1.140
-
benzylisoquinoline
-
n-methylated
- japonica
- coptis
- isoquinoline
- norreticuline
-
s-reticuline
- n-methyltransferases
- norcoclaurine
- poppy
- scoulerine
- somniferum
-
3\'-hydroxylase
-
4\'omt
- magnoflorine
-
6-o-methyltransferase
-
opium
-
papaver
- tetrahydroisoquinoline
-
aporphine
- medicine
- biotechnology
Reaction
Synonyms
(S)-Coclaurine N-methyltransferase, 6OMT, CjCNMT, CMT, CNMT, CNMT protein, coclaurine N-methyltransferase, coclaurine-N-methyltransferase, FspC, S-adenosyl-L-methionine:coclaurine N-methyltransferase
ECTree
2.1.1.140 (S)-coclaurine-N-methyltransferaseAdvanced search results
results (
results (Specific Activity
Specific Activity on EC 2.1.1.140 - (S)-coclaurine-N-methyltransferase
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SPECIFIC ACTIVITY [µmol/min/mg] 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
0.000096
0.0001
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.0003
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00065
Thalictrum glaucum
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00066
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.0007
Corydalis vaginans
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00075
Bocconia cordata
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.00077
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.0013
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.002
0.003
Tinospora fragosa
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
additional information
0.000096
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enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
0.000096
-
enzyme extract of cell culture, (S)-coclaurine, (R)coclaurine is not methylated
additional information
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(S)-corytuberine conversion to magnoflorine shown by crude enzyme preparation of recombinant CNMT protein, magnoflorine biosynthesis by CYP80G2 protein and CjCNMT via (S)-corytuberine as an intermediate in vivo, CjCNMT protein catalyzes the crucial N-methylation step from (S)-coclaurine to (S)-N-methylcoclaurine in (S)-reticuline biosynthesis
additional information
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analysis of inducible expression systems, production of recombinant CMT protein from PprpB promoter 2fold to 4fold higher than that from PT7 promoter, cell growth not reduced after expression form PprpB promoter even when highly induced, propionate-inducible system efficient for protein overproduction that result in growth inhibition, auto-induction experiments performed and protein production monitored
additional information
phylogenetic analysis reveales a monophyletic clade for (S)-coclaurine N-methyltransferase (CNMT) and (S)-tetrahydroprotoberberine cis-N-methyltransferase (TNMT), 48% identity of amino acid sequence, downstream metabolic role suggests that TNMT derives from gene duplication after more ancient recruitment of CNMT, phylogenetic relationship between CNMT and TNMT provides new insights into evolutionary recruitment of enzymes into plant alkaloid pathways
additional information
-
phylogenetic analysis reveales a monophyletic clade for (S)-coclaurine N-methyltransferase (CNMT) and (S)-tetrahydroprotoberberine cis-N-methyltransferase (TNMT), 48% identity of amino acid sequence, downstream metabolic role suggests that TNMT derives from gene duplication after more ancient recruitment of CNMT, phylogenetic relationship between CNMT and TNMT provides new insights into evolutionary recruitment of enzymes into plant alkaloid pathways
