1.3.3.5: bilirubin oxidase
This is an abbreviated version!
For detailed information about bilirubin oxidase, go to the full flat file.
Word Map on EC 1.3.3.5
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1.3.3.5
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electrode
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oxidases
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biofuel
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cathode
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ceruloplasmin
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myrothecium
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anode
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laccases
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verrucaria
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biocathode
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ferroxidase
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trinuclear
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abts
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laccase-like
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dioxygen
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bioanode
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electrocatalytic
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bioelectrocatalytic
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2,6-dimethoxyphenol
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biliverdin
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cuprous
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multi-copper
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syringaldazine
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copa
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copper-binding
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four-electron
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mniii
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self-powered
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aceruloplasminemia
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hephaestin
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p-phenylenediamine
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manganeseii
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bioelectrodes
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open-circuit
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membrane-less
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cupredoxins
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analysis
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electrocatalysts
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manganese-oxidizing
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methionine-rich
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diazo
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biodevice
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energy production
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medicine
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diagnostics
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biotechnology
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synthesis
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environmental protection
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industry
- 1.3.3.5
-
electrode
- oxidases
-
biofuel
-
cathode
- ceruloplasmin
- myrothecium
-
anode
- laccases
- verrucaria
-
biocathode
- ferroxidase
-
trinuclear
- abts
-
laccase-like
- dioxygen
-
bioanode
-
electrocatalytic
-
bioelectrocatalytic
- 2,6-dimethoxyphenol
- biliverdin
-
cuprous
-
multi-copper
- syringaldazine
- copa
-
copper-binding
-
four-electron
-
mniii
-
self-powered
-
aceruloplasminemia
- hephaestin
- p-phenylenediamine
-
manganeseii
-
bioelectrodes
-
open-circuit
-
membrane-less
- cupredoxins
- analysis
-
electrocatalysts
-
manganese-oxidizing
-
methionine-rich
-
diazo
-
biodevice
- energy production
- medicine
- diagnostics
- biotechnology
- synthesis
- environmental protection
- industry
Reaction
2 bilirubin + = 2 biliverdin + 2 H2O
Synonyms
bilirubin oxidase, bilirubin oxidase M-1, bilirubin:oxygen oxidoreductase, blue Cu enzyme, BOD, BODx, BOX, BPUM_0542, copper oxidase, CotA, MCO, multicopper oxidase, MvBO, MvBOD, oxidase, bilirubin
ECTree
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Specific Activity
Specific Activity on EC 1.3.3.5 - bilirubin oxidase
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2.83
214.3
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purified enzyme after anion exchange chromatography step, pH 7.5, 25°C
24
25.4
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purified enzyme with substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH 6.0, 24°C
additional information
2.83
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commercial preparation, pH not specified in the publication, temperature not specified in the publication
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3.3 units/ml, 1 unit is defined as the equivalent amount of enzyme that reduces DELTA A at 440 nm by 1.05/min, enzyme activity in crude extracts
additional information
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206 units/A280, 1 unit is defined as the equivalent amount of enzyme that reduces DELTA A at 440 nm by 1.05/min, enzyme activity in crude extracts
additional information
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no bilirubin oxidase activity of mutant C457S and mutant D105N
additional information
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relative activity 0.09, 0.2 g/l BOD stored in 0.5 g/l poly[ethyleneimine], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.09, 0.2 g/l BOD stored in 2 g/l poly[ethyleneimine], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.17, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M H2PO4--HPO42- buffer system, no additive
additional information
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relative activity 0.17, 0.2 g/l BOD stored in 0.1 M H2PO4--HPO42- buffer system, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.19, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M MOPS0-MOPS- buffer system, no additive
additional information
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relative activity 0.22, 0.2 g/l BOD stored in 0.5 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,6-hexanediylimino(1-oxo-1,2-ethanediyl)(dimethylimino)-1,6-hexanediyl], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.22, 0.2 g/l BOD stored in 0.5 g/l poly[(dimethylimino)-1,6-hexanediyl], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.22, 0.2 g/l BOD stored in 2 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,6-hexanediylimino(1-oxo-1,2-ethanediyl)(dimethylimino)-1,6-hexanediyl], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.22, 0.2 g/l BOD stored in 2 g/l poly[(dimethylimino)-1,6-hexanediyl], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.23, 0.2 g/l BOD stored in 0.5 g/l poly(diallydimethylammonium), 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.23, 0.2 g/l BOD stored in 2 g/l poly(diallydimethylammonium), 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.25, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M H2PO4--HPO42- buffer system, 0.5 g/l poly[oxyethylene(dimethylimino)propyl(dimethylimino)ethylene]
additional information
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relative activity 0.26, 0.2 g/l BOD stored in 0.5 g/l poly[metacrylpropyltrimethylammonium], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.26, 0.2 g/l BOD stored in 2 g/l poly[metacrylpropyltrimethylammonium], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.27, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M H2PO4--HPO42- buffer system, 0.5 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,2-ethanediylimino(1-oxo-1,2-ethanediyl)-(dimethylimino)-1,3-propanediyl]
additional information
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relative activity 0.27, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M H2PO4--HPO42- buffer system, 0.5 g/l poly[bis(2-chloroethyl)ether-alt-1,3-bis[3-(dimethylimino)propyl]urea]
additional information
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relative activity 0.27, 0.2 g/l BOD stored in 0.5 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,2-ethanediylimino(1-oxo-1,2-ethanediyl)(dimethylimino)-1,3-propanediyl], 0.1 M H2PO4--HPO42- buffer system, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.27, 0.2 g/l BOD stored in 0.5 g/l poly[bis(2-chloroethyl)ether-alt-1,3-bis[3-(dimethylimino)propyl]urea], 0.1 M H2PO4--HPO42- buffer system, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.28, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M H2PO4--HPO42- buffer system, 0.5 g/l poly(dimethylamine-co-epichrohydrin)
additional information
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relative activity 0.28, 0.2 g/l BOD stored in 0.5 g/l poly(dimethylamine-co-epichrohydrin), 0.1 M H2PO4--HPO42- buffer system, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.28, 0.2 g/l BOD stored in 0.5 g/l poly[allyamine], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.28, 0.2 g/l BOD stored in 2 g/l poly[allyamine], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.30, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M MOPS0-MOPS- buffer system, 0.5 g/l poly[bis(2-chloroethyl)ether-alt-1,3-bis[3-(dimethylimino)propyl]urea]
additional information
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relative activity 0.30, 0.2 g/l BOD stored in 0.5 g/l poly[bis(2-chloroethyl)ether-alt-1,3-bis[3-(dimethylimino)propyl]urea], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.30, 0.2 g/l BOD stored in 2 g/l poly[bis(2-chloroethyl)ether-alt-1,3-bis[3-(dimethylimino)propyl]urea], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.31, 0.2 g/l BOD stored in 0.5 g/l poly[lysine], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.31, 0.2 g/l BOD stored in 2 g/l poly[lysine], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.38, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M MOPS0-MOPS- buffer system, 0.5 g/l poly(dimethylamine-co-epichrohydrin)
additional information
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relative activity 0.38, 0.2 g/l BOD stored in 0.5 g/l poly(dimethylamine-co-epichrohydrin), 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.38, 0.2 g/l BOD stored in 2 g/l poly(dimethylamine-co-epichrohydrin), 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.40, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M MOPS0-MOPS- buffer system, 0.5 g/l poly[oxyethylene(dimethylimino)propyl(dimethylimino)ethylene]
additional information
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relative activity 0.43, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M MOPS0-MOPS- buffer system, 0.5 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,2-ethanediylimino(1-oxo-1,2-etanediyl)-(dimethylimino)-1,3-propanediyl]
additional information
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relative activity 0.43, 0.2 g/l BOD stored in 0.5 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,2-ethanediylimino(1-oxo-1,2-ethanediyl)(dimethylimino)-1,3-propanediyl], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.43, 0.2 g/l BOD stored in 2 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,2-ethanediylimino(1-oxo-1,2-ethanediyl)(dimethylimino)-1,3-propanediyl], 0.1 M MOPS-NaOH solution, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.51, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M BisTrisH+-BisTris0 buffer system, no additive
additional information
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relative activity 0.51, 0.2 g/l BOD stored in 0.1 M BisTrisH+-BisTris0 buffer system, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.58, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M BisTrisH+-BisTris0 buffer system, 0.5 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,2-ethanediylimino(1-oxo-1,2-etanediyl)-(dimethylimino)-1,3-propanediyl]
additional information
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relative activity 0.58, 0.2 g/l BOD stored in 0.5 g/l poly[(dimethylimino)(2-oxo-1,2-ethanediyl)imino-1,2-ethanediylimino(1-oxo-1,2-ethanediyl)(dimethylimino)-1,3-propanediyl], 0.1 M BisTrisH+-BisTris0 buffer system, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.64, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M BisTrisH+-BisTris0 buffer system, 0.5 g/l poly[bis(2-chloroethyl)ether-alt-1,3-bis[3-(dimethylimino)propyl]urea]
additional information
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relative activity 0.64, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M BisTrisH+-BisTris0 buffer system, 0.5 g/l poly[oxyethylene(dimethylimino)propyl(dimethylimino)ethylene]
additional information
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relative activity 0.64, 0.2 g/l BOD stored in 0.5 g/l poly[bis(2-chloroethyl)ether-alt-1,3-bis[3-(dimethylimino)propyl]urea], 0.1 M BisTrisH+-BisTris0 buffer system, pH 7.0, 30°C, 2 days
additional information
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relative activity 0.89, 0.2 g/l BOD stored at 30°C for 2 days in 0.1 M BisTrisH+-BisTris0 buffer system, 0.5 g/l poly(dimethylamine-co-epichrohydrin)
additional information
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relative activity 0.89, 0.2 g/l BOD stored in 0.5 g/l poly(dimethylamine-co-epichrohydrin), 0.1 M BisTrisH+-BisTris0 buffer system, pH 7.0, 30°C, 2 days
additional information
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0.67 units/ml, 1 unit is defined as the equivalent amount of enzyme that reduces DELTA A at 440 nm by 1.05/min, enzyme activity in crude extracts
additional information
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0.02 units/ml, 1 unit is defined as the equivalent amount of enzyme that reduces DELTA A at 440 nm by 1.05/min, enzyme activity in crude extracts