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1.3.3.5: bilirubin oxidase

This is an abbreviated version!
For detailed information about bilirubin oxidase, go to the full flat file.

Word Map on EC 1.3.3.5

Reaction

2 bilirubin +

O2
= 2 biliverdin + 2 H2O

Synonyms

bilirubin oxidase, bilirubin oxidase M-1, bilirubin:oxygen oxidoreductase, blue Cu enzyme, BOD, BODx, BOX, BPUM_0542, copper oxidase, CotA, MCO, multicopper oxidase, MvBO, MvBOD, oxidase, bilirubin

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.5 bilirubin oxidase

General Stability

General Stability on EC 1.3.3.5 - bilirubin oxidase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
after an incubation of 8 days at physiological conditions, the enzyme loses 15% of its activity
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after the tenth cycle (2 months of storage), the enzyme retains more than 50% of its initial activity, while for homogeneous solution, the half live of the enzyme is 3 weeks
bilirubin oxidase-coated carbon cathode operates for more than 1 week at 37°C in a glucose-containing physiological buffer solution. The cathode is short-lived in serum, losing its electrocatalytic activity in a few hours, caused by a product of the reaction of urate and dissolved oxygen. Exclusion of urate, by application of Nafion(TM) film in the cathode, improves the stability in serum
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BOD can undergo non-catalytic direct electron transfer between enzyme and carbon electrode and retains its catalytic activity, 1%-2% of the immobilized BOD (as a first monolayer on the electrode surface) participates in direct electrical communication with a carbon electrode
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immobilized enzyme, increased heat stability
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multi-walled carbon nanotube modification of glassy carbon electrodes strongly enhances the oxygen reduction of bilirubin oxidase compared to unmodified electrodes, the quasi-reversible redox reaction might be attributed to the trinuclear T2/T3 cluster of BOD, a BOD-multi-walled carbon nanotube-modified electrode retains 70% of the initial activity for oxygen reduction after 4 days
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the recombinant enzyme shows high tolerance towards urea
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