Cloned (Comment) | Organism |
---|---|
Pichia pastoris GS115 transformed using the pPICBO derivative linearized with Bpu1102I | Albifimbria verrucaria |
Protein Variants | Comment | Organism |
---|---|---|
C457S | can react with dioxygen, affords reaction intermediate I with absorption maxima at 340, 470, and 675 nm | Albifimbria verrucaria |
D105A | exhibits 7.5% bilirubin oxidase activity compared to the wild-type enzyme, indicating that Asp105 conserved in all multi-copper oxidases donates a proton to reaction intermediates I and II | Albifimbria verrucaria |
D105E | exhibits 46% bilirubin oxidase activity compared to the wild-type enzyme, indicating that Asp105 conserved in all multi-copper oxidases donates a proton to reaction intermediates I and II | Albifimbria verrucaria |
D105N | does not react with dioxygen | Albifimbria verrucaria |
M467G | with modified spectroscopic properties and redox potential, affords reaction intermediate II with absorption maxima at 355 and 450 nm | Albifimbria verrucaria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
all mutants expressed in Pichia pastoris, SDS-PAGE | Albifimbria verrucaria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Albifimbria verrucaria | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Albifimbria verrucaria |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
no bilirubin oxidase activity of mutant C457S and mutant D105N | Albifimbria verrucaria |
0.3 | - |
ferrocyanide oxidase activity of mutant M467G | Albifimbria verrucaria |
1.2 | - |
bilirubin oxidase activity of mutant D105A | Albifimbria verrucaria |
11 | - |
bilirubin oxidase activity of mutant D105E | Albifimbria verrucaria |
24 | - |
bilirubin oxidase activity of authentic enzyme | Albifimbria verrucaria |
24 | - |
bilirubin oxidase activity of recombinant enzyme | Albifimbria verrucaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bilirubin + O2 | - |
Albifimbria verrucaria | biliverdin + H2O | - |
? | |
ferrocyanide + O2 | - |
Albifimbria verrucaria | ? | - |
? |