Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris. The cDNA encoding bilirubin oxidase is cloned into the Pichia pastoris expression vector pPIC9K under the control of the alcohol oxidase 1 promoter and its protein product is secreted using the Saccharomyces alpha-mating factor signal sequence | Albifimbria verrucaria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
bilirubin | pH 8.0, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
0.12 | - |
bilirubin | pH 8.0, authentic bilirubin oxidase | Albifimbria verrucaria | |
0.14 | - |
ditaurobilirubin | pH 5.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
0.25 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
0.34 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
0.37 | - |
ditaurobilirubin | pH 5.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
9.6 | - |
p-phenylenediamine | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
9.8 | - |
p-phenylenediamine | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | multicopper oxidase contains 4 copper ions per protein molecule. Cu-binding sites are not affected by differences in carbohydrate content and the N-terminal extension of four amino acid residues | Albifimbria verrucaria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
x * 66000, SDS-PAGE | Albifimbria verrucaria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Albifimbria verrucaria | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the recombinant enzyme expressed in Pichia pastoris contains non-native type N-linked sugar chains | Albifimbria verrucaria |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Albifimbria verrucaria |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57.2 | - |
- |
Albifimbria verrucaria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dihydroxynaphthalene + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
1,5-dihydroxynaphthalene + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonate) + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
bilirubin + O2 | - |
Albifimbria verrucaria | biliverdin + H2O | - |
? | |
ditaurobilirubin + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
N,N-dimethyl-p-phenylenediamine + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
o-aminophenol + O2 | - |
Albifimbria verrucaria | ? | - |
? | |
p-phenylenediamine + O2 | - |
Albifimbria verrucaria | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66000, SDS-PAGE | Albifimbria verrucaria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Albifimbria verrucaria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life of the recombinant enzyme expressed in Escherichia coli is 90 min, half-life of authentic enzyme is 15 min | Albifimbria verrucaria |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
60 | - |
p-phenylenediamine | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
90 | - |
p-phenylenediamine | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
94.3 | - |
ditaurobilirubin | pH 5.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
115 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, authentic bilirubin oxidase | Albifimbria verrucaria | |
117 | - |
bilirubin | pH 8.0, authentic bilirubin oxidase | Albifimbria verrucaria | |
164 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) | pH 6.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
200 | - |
bilirubin | pH 8.0, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria | |
233 | - |
ditaurobilirubin | pH 5.5, recombinant enzyme from Pichia pastoris | Albifimbria verrucaria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Albifimbria verrucaria |