1.13.11.40: arachidonate 8-lipoxygenase

This is an abbreviated version!
For detailed information about arachidonate 8-lipoxygenase, go to the full flat file.

Word Map on EC 1.13.11.40

1.13.11.40

lipoxygenases

coral

allene

plexaura

homomalla

8r-hpete

epidermis-type

whip

12r-lox

8-hydroxyeicosatetraenoic

medicine

Reaction

(5Z,9E,11Z,14Z)-(8R)-8-hydroperoxyicosa-5,9,11,14-tetraenoate

Synonyms

8(R)-lipoxygenase, 8-lipoxygenase, 8-LOX, 8R-lipoxygenase, 8R-LOX, 8S-lipoxygenase, 8S-LOX, allene oxide synthase-lipoxygenase protein, arachidonic acid C-8 lipoxygenase, eicosapentaenoic 8R-lipoxygenase, LOX-1, More

ECTree

1 Oxidoreductases

1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

1.13.11 With incorporation of two atoms of oxygen

1.13.11.40 arachidonate 8-lipoxygenase

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Results	in table
2	Activating Compound
7	AA Sequence
2	Application
1	CAS Registry Number
11	Cloned(Commentary)
5	Crystallization (Commentary)
4	General Information
2	General Stability
7	Inhibitors
4	kcat/KM [mM/s]
17	KM Value [mM]
3	Localization
4	Metals/Ions
2	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
22	Organism
4	Pathway
20	PDB ID
5	pH Optimum
1	pH Range
1	Posttranslational Modification
26	Protein Variants
9	Purification (Commentary)
1	Reaction
4	Reaction Type
22	Reference
7	Source Tissue
2	Specific Activity [micromol/min/mg]
3	Storage Stability
51	Substrates and Products (Substrate)
2	Subunits
22	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
17	Turnover Number [1/s]

Purification

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Purification on EC 1.13.11.40 - arachidonate 8-lipoxygenase

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2 entries

Ni-NTA-agarose column chromatography

Mus musculus

671715

nickel-nitrilotriacetic acid-agarose chromatography, DE52 column chromatography, and Mono Q column chromatography

on Ni column

partial

Pseudoplexaura porosa

439396

recombinant enzyme

Mus musculus

439401

recombinant N-terminally His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)

Plexaura homomalla

O16025

742865

three-step purification, can be completed in two days, yields about 10 mg enzyme per 1 l of expression media, highly purified enzyme

Plexaura homomalla

Q27901

765335