1.13.11.40: arachidonate 8-lipoxygenase

This is an abbreviated version!
For detailed information about arachidonate 8-lipoxygenase, go to the full flat file.

Reaction

Synonyms

8(R)-lipoxygenase, 8-lipoxygenase, 8-LOX, 8R-lipoxygenase, 8R-LOX, 8S-lipoxygenase, 8S-LOX, allene oxide synthase-lipoxygenase protein, arachidonic acid C-8 lipoxygenase, eicosapentaenoic 8R-lipoxygenase, LOX-1, More

ECTree

     1 Oxidoreductases

         1.13 Acting on single donors with incorporation of molecular oxygen (oxygenases)

             1.13.11 With incorporation of two atoms of oxygen

                1.13.11.40 arachidonate 8-lipoxygenase

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
2	Activating Compound
7	AA Sequence
2	Application
1	CAS Registry Number
11	Cloned(Commentary)
5	Crystallization (Commentary)
4	General Information
2	General Stability
7	Inhibitors
4	kcat/KM [mM/s]
17	KM Value [mM]
3	Localization
4	Metals/Ions
2	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
22	Organism
4	Pathway
20	PDB ID
5	pH Optimum
1	pH Range
1	Posttranslational Modification
26	Protein Variants
9	Purification (Commentary)
1	Reaction
4	Reaction Type
22	Reference
7	Source Tissue
2	Specific Activity [micromol/min/mg]
3	Storage Stability
51	Substrates and Products (Substrate)
2	Subunits
22	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
17	Turnover Number [1/s]

Cloned

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Cloned on EC 1.13.11.40 - arachidonate 8-lipoxygenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide Go to Cloned (commentary) Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

-

Plexaura homomalla

O16025

702338

expressed in Escherichia coli BL21(DE3) cells

Plexaura homomalla

-

674505

expressed in Escherichia coli strain BL21 (DE3)

Mus musculus

-

671715

expression and establishment of a doxycycline-inducible Tet-On gene expression system in the premalignant mouse keratinocyte cell line 308

Mus musculus

-

688221

expression in Escherichia coli

Plexaura homomalla

Q27901

765335

expression in HeLa cells

Plexaura homomalla

-

660365

expression in Sf9 cells using a baculovirus expression system

Euphausia pacifica

A0A7G1H6X8

765778

full-length cDNA cloned by PCR

Plexaura homomalla

-

439399

ligated into the pQE-9 plasmid and expressed as N-terminal His-tag fusion protein in Escherichia coli (XL-1 Blue)

Mus musculus

-

702435

recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)

Plexaura homomalla

O16025

742865

skin 8-lipoxygenase expressed in COS-7 cells by transient transfection of its cDNA, amplified by PCR, in pEF-BOS, also expressed in Escherichia coli system using pQE-32

Mus musculus

-

439401