EC Number   |
Protein Variants |
Reference |
|---|
   3.1.1.22 | more |
generation of a set of phaZ gene deletion strains, i.e. DELTAphaZ1, DELTAphaZ2, DELTAphaZ3, DELTAphaZ1DELTAphaZ2, DELTAphaZ1DELTAphaZ3, DELTAphaZ2DELTAphaZ3, and DELTAphaZ1DELTAphaZ2DELTAphaZ3 from strain H16. All mutant strains are unable to produce poly(hydroxybutyrate) |
750947 |
| 3.1.1.22 | more |
recombinant Cupriavidus necator strain KNK-005 harbors an NSDG mutant of the PHA synthase gene (phaCAc) from Aeromonas caviae. Deletion of phaZ1 (EC 3.1.1.75, UniProt ID Q0KCI0) and phaZ2 has a significant and slight attenuating effect, respectively, on the reduction in polyhydroxyalkanoate (PHA) content of KNK-005 cells. Regardless of the PHA consumption, its molecular weight does not decrease. The DELTAphaZ1DELTAphaZ2DELTAphaZ6 triple mutant of KNK-005, 005dZ126, is a promising strain capable of producing ultra-high-molecular-weight poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) of ultra-high-molecular-weight and barely degrades PHBHHx and polyhydroxyalkanoate enzymatically intracellularly |
751154 |
| 3.1.1.22 | S102A |
site-directed mutagenesis, inactive mutant |
650922 |
| 3.1.1.22 | S191A |
site-directed mutagenesis, unaltered activity compared to the wild-type enzyme |
652588 |
| 3.1.1.22 | S218A |
site-directed mutagenesis, slightly decreased activity compared to the wild-type enzyme |
652588 |
| 3.1.1.22 | S306A |
site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme |
652588 |
| 3.1.1.22 | S356A |
site-directed mutagenesis, slightly increased activity compared to the wild-type enzyme |
652588 |
| 3.1.1.22 | S359A |
site-directed mutagenesis, inactive mutant |
652588 |
| 3.1.1.22 | S630A |
site-directed mutagenesis, increased activity compared to the wild-type enzyme |
652588 |
