EC Number   |
Protein Variants |
Reference |
|---|
   3.2.2.23 | F111A |
the mutant displays a significant increase in the average diffusion constant compared to the wild-type protein with no enzymatic activity on DNA containing 8-oxoguanine residues opposite cytosine. The mutant has little or no ability to form a Schiff base with 8-oxoguanine residues opposite cytosine or 5,6-dihydrouracil opposite guanine compared to wild type enzyme |
716394 |
| 3.2.2.23 | H71A |
severely compromised in turnover of oligonucleotides with 8-oxoguanosine opposie cytosine, but show turnover rates comparable to wild-type on abasic-site containing DNA |
678197 |
| 3.2.2.23 | H89A |
selective diminition of the rate of excision of 8-oxoguanine |
669289 |
| 3.2.2.23 | H89A/R109A |
about 10fold increase in KM-value |
669289 |
| 3.2.2.23 | K155A |
effect of mutation on specificity, mutant with very low activity, kinetic study |
646944 |
| 3.2.2.23 | K155A |
mutant enzyme with decreased N-glycosylase and increased AP lyase activity, it dissociates prematurely from the covalent enzyme-DNA complex leading to a higher turnover number for DNA containing an AP site |
646946 |
| 3.2.2.23 | K155A |
mutant with 50fold decreased activity with 7-hydro-8-oxoguanine-DNA as substrate, only 3-4fold decreased activity with 7-methylformamidopyrimidine-DNA, increased AP lyase activity |
646945 |
| 3.2.2.23 | K155A |
mutant with reduced 8-oxoguanine-DNA but unchanged Fapy-DNA glycosylase activity |
-, 646950 |
| 3.2.2.23 | K217T |
selective reduction of the ability to excise 8-oxoguanine from DNA |
669289 |
| 3.2.2.23 | K57A |
mutant with about 15% of wild-type activity in both N-glycosylase and AP lyase activity |
646946 |
