Cloned (Comment) | Organism |
---|---|
expression of K155A mutant and wild-type Fpg in Escherichia coli BL21(DE3) and BLR(DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K155A | mutant with 50fold decreased activity with 7-hydro-8-oxoguanine-DNA as substrate, only 3-4fold decreased activity with 7-methylformamidopyrimidine-DNA, increased AP lyase activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000009 | - |
DNA | pH 7.5, 20°C, 24-oligomer DNA containing apurinic/apyrimidinic sites, lyase activity, K155A mutant Fpg | Escherichia coli | |
0.0000069 | - |
DNA containing 7-hydro-8-oxoguanine residues | pH 7.5, 37°C, 24-oligomer, wild-type Fpg | Escherichia coli | |
0.0000075 | - |
DNA containing 7-hydro-8-oxoguanine residues | pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg | Escherichia coli | |
0.000019 | - |
DNA | pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg | Escherichia coli | |
0.000023 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.5, 37°C, 23-oligomer, wild-type Fpg | Escherichia coli | |
0.000029 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
1 * 35000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | removes oxidized purines from oxidatively damaged DNA | ? | - |
? | |
DNA + H2O | Escherichia coli | DNA base excision repair enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P50465 | strain 5H11S | - |
Purification (Comment) | Organism |
---|---|
wild-type and K155A mutant Fpg, expressed in Escherichia coli BLR(DE3) | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.054 | - |
polymeric DNA containing ring-opened N7-methylguanine residues as substrate, wild-type Fpg | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions | Escherichia coli | ? | - |
? | |
DNA + H2O | removes oxidized purines from oxidatively damaged DNA | Escherichia coli | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | ? | - |
? | |
DNA + H2O | DNA base excision repair enzyme | Escherichia coli | ? | - |
? | |
DNA containing 2'-deoxy-8-oxonebularine residues + H2O | 23mer oligonucleotide containing a single site, very poor substrate | Escherichia coli | ? | - |
? | |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O | FapyGua | Escherichia coli | 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O | FapyAde | Escherichia coli | 4,6-diamino-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 7-hydro-8-oxoguanine residues + H2O | 24-oligomer, Lys-155 directly interacts with the C8 oxygen of 8-oxopurines involving proton transfer or transient formation of an ion pair between enzyme and substrate, mechanism | Escherichia coli | DNA + 7-hydro-8-oxoguanine | - |
? | |
DNA containing 7-hydro-8-oxoguanine residues + H2O | DNA glycosylase/AP lyase activity | Escherichia coli | DNA + 7-hydro-8-oxoguanine | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | 23-oligomer | Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 35000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000383 | - |
DNA containing 7-hydro-8-oxoguanine residues | pH 7.5, 37°C, 24-oligomer, K155A mutant Fpg | Escherichia coli | |
0.0012 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.5, 37°C, 23-oligomer, K155A mutant Fpg | Escherichia coli | |
0.00383 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.5, 37°C, 23-oligomer, wild-type Fpg | Escherichia coli | |
0.0185 | - |
DNA containing 7-hydro-8-oxoguanine residues | pH 7.5, 37°C, 24-oligomer, wild-type Fpg | Escherichia coli | |
0.0568 | - |
DNA | pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, wild-type Fpg | Escherichia coli | |
0.0883 | - |
DNA | pH 7.5, 20°C, 24-oligomer DNA containing AP sites, lyase activity, K155A mutant Fpg | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |