Protein Variants | Comment | Organism |
---|---|---|
K155A | mutant enzyme with decreased N-glycosylase and increased AP lyase activity, it dissociates prematurely from the covalent enzyme-DNA complex leading to a higher turnover number for DNA containing an AP site | Escherichia coli |
K57A | mutant with about 15% of wild-type activity in both N-glycosylase and AP lyase activity | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for DNA substrate is affected by the base opposite the lesion | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | with apurinic/apyrimidinic lyase activity, catalyzes beta and delta elimination reactions | Escherichia coli | ? | - |
? | |
DNA + H2O | role of Lys-155 for substrate binding and product release, AP lyase mechanism | Escherichia coli | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | ? | - |
? | |
DNA containing 2,6-diamino-4-hydroxy-5-formamidopyrimidine residues + H2O | FapyGua | Escherichia coli | 2,6-diamino-4-hydroxy-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 4,6-diamino-5-formamidopyrimidine residues + H2O | FapyAde | Escherichia coli | 4,6-diamino-5-formamidopyrimidine + DNA | - |
? | |
DNA containing 7-hydro-8-oxoguanine residues + H2O | - |
Escherichia coli | DNA + 7-hydro-8-oxoguanine | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | - |
Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |