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EC Number Protein Variants Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.4.25S36A site directed mutagenesis 676227
Display the word mapDisplay the reaction diagram Show all sequences 2.7.4.25S36A the S36A substitution mainly changes the Km value for the two natural substrates, which increases by a factor of 70 (CMP) and 37 (dCMP) compared with the parent molecule and decreases kcat of 1.6fold (CMP) and 7.4fold (dCMP) with respect to the wild type enzyme 692282
Display the word mapDisplay the reaction diagram Show all sequences 2.7.4.25S36A the side chains of Arg110 cannot establish hydrogen bonds with UMP, and its substitution by hydrophobic amino acids simultaneously affects the Km of CMP/dCMP and the kcat value 692282
Display the word mapDisplay the reaction diagram Show all sequences 2.7.4.25V164E substitution of Val164 by a Glu residue apparently does not affect the catalytic properties of Escherichia coli CMP kinase 645154
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