BRENDA - Enzyme Database
show all sequences of 2.7.4.25

CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases

Bucurenci, N.; Sakamoto, H.; Briozzo, P.; Palibroda, N.; Serina, L.; Sarfati, R.S.; Labesse, G.; Briand, G.; Danchin, A.; Barzu, O.; Gilles, A.M.; J. Biol. Chem. 271, 2856-2862 (1996)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli K-12
Crystallization (Commentary)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20C
Escherichia coli K-12
hanging drop vapor diffusion technique
Escherichia coli
Engineering
Amino acid exchange
Commentary
Organism
V164E
substitution of Val164 by a Glu residue apparently does not affect the catalytic properties of Escherichia coli CMP kinase
Escherichia coli K-12
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no inhibitors of the enzyme
Escherichia coli K-12
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
CMP
reaction with ATP
Escherichia coli
0.035
-
CMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.038
-
ATP
pH 7.4, reaction with CMP
Escherichia coli
0.038
-
ATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.087
-
dATP
pH 7.4, reaction with CMP
Escherichia coli
0.087
-
dATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.094
-
dCMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.094
-
dGMP
reaction with ATP
Escherichia coli
0.36
-
1-beta-D-arabinofuranosylcytosine 5'-phosphate
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.36
-
araCMP
reaction with ATP
Escherichia coli
0.64
-
GTP
reaction with CMP
Escherichia coli
0.64
-
GTP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.87
-
ITP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.93
-
UMP
reaction with ATP
Escherichia coli
0.93
-
UMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
1.46
-
dUMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24620
-
electrospray ionization mass spectrometry
Escherichia coli K-12
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Escherichia coli K-12
P0A6I0
strain NM554
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
blue Sepharose column chromatography and Ultrogel AcA54 gel filtration
Escherichia coli K-12
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
645154
Escherichia coli K-12
ADP + 1-beta-D-arabinofuranosylcytosine 5'-diphosphate
-
-
-
?
ATP + 2-thiouridine 5'-monophosphate
2-thiouridine 5'-monophosphate is a poor substrate
645154
Escherichia coli K-12
ADP + 2-thiouridine 5'-diphosphate
-
-
-
?
ATP + ara-CMP
-
645154
Escherichia coli
ADP + ara-CDP
-
-
-
?
ATP + CMP
-
645154
Escherichia coli
ADP + CDP
-
-
-
?
ATP + CMP
-
645154
Escherichia coli K-12
ADP + CDP
-
-
-
?
ATP + dCMP
-
645154
Escherichia coli
ADP + dCDP
-
-
-
?
ATP + dCMP
-
645154
Escherichia coli K-12
ADP + dCDP
-
-
-
?
ATP + dUMP
dUMP is a poor substrate
645154
Escherichia coli K-12
ADP + dUDP
-
-
-
?
ATP + UMP
-
645154
Escherichia coli
ADP + UDP
-
-
-
?
ATP + UMP
UMP is a poor substrate
645154
Escherichia coli K-12
ADP + UDP
-
-
-
?
CTP + CMP
the activity with CTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
CDP + CDP
-
-
-
?
dATP + CMP
-
645154
Escherichia coli
dADP + CDP
-
-
-
-
dATP + CMP
-
645154
Escherichia coli K-12
dADP + CDP
-
-
-
?
dCTP + CMP
the activity with dCTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dCDP + CDP
-
-
-
?
dUTP + CMP
the activity with dUTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dUDP + CDP
-
-
-
?
GTP + CMP
-
645154
Escherichia coli
GDP + CDP
-
-
-
?
GTP + CMP
-
645154
Escherichia coli K-12
GDP + CDP
-
-
-
?
ITP + CMP
ITP is a poor substrate
645154
Escherichia coli K-12
IDP + CDP
-
-
-
?
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no substrates
645154
Escherichia coli K-12
?
-
-
-
-
UTP + CMP
the activity with UTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
UDP + CDP
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
2 * 000, SDS-PAGE
Escherichia coli K-12
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
52
-
CMP kinase is half-inactivated at 52C
Escherichia coli K-12
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia coli K-12
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Escherichia coli K-12
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Escherichia coli K-12
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop vapor diffusion method, using 0.4 M ammonium sulfate in 50 mM Tris-HCl buffer (pH 7.4), at 20C
Escherichia coli K-12
hanging drop vapor diffusion technique
Escherichia coli
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
V164E
substitution of Val164 by a Glu residue apparently does not affect the catalytic properties of Escherichia coli CMP kinase
Escherichia coli K-12
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no inhibitors of the enzyme
Escherichia coli K-12
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
CMP
reaction with ATP
Escherichia coli
0.035
-
CMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.038
-
ATP
pH 7.4, reaction with CMP
Escherichia coli
0.038
-
ATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.087
-
dATP
pH 7.4, reaction with CMP
Escherichia coli
0.087
-
dATP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.094
-
dCMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.094
-
dGMP
reaction with ATP
Escherichia coli
0.36
-
1-beta-D-arabinofuranosylcytosine 5'-phosphate
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.36
-
araCMP
reaction with ATP
Escherichia coli
0.64
-
GTP
reaction with CMP
Escherichia coli
0.64
-
GTP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.87
-
ITP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
0.93
-
UMP
reaction with ATP
Escherichia coli
0.93
-
UMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
1.46
-
dUMP
in 50 mM Tris-HCl (pH 7.4), 50 mM KCl, 2 mM MgCl2, 1 mM phosphoenolpyruvate, 0.2 mM NADH, at 30C
Escherichia coli K-12
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
24620
-
electrospray ionization mass spectrometry
Escherichia coli K-12
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
blue Sepharose column chromatography and Ultrogel AcA54 gel filtration
Escherichia coli K-12
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
645154
Escherichia coli K-12
ADP + 1-beta-D-arabinofuranosylcytosine 5'-diphosphate
-
-
-
?
ATP + 2-thiouridine 5'-monophosphate
2-thiouridine 5'-monophosphate is a poor substrate
645154
Escherichia coli K-12
ADP + 2-thiouridine 5'-diphosphate
-
-
-
?
ATP + ara-CMP
-
645154
Escherichia coli
ADP + ara-CDP
-
-
-
?
ATP + CMP
-
645154
Escherichia coli
ADP + CDP
-
-
-
?
ATP + CMP
-
645154
Escherichia coli K-12
ADP + CDP
-
-
-
?
ATP + dCMP
-
645154
Escherichia coli
ADP + dCDP
-
-
-
?
ATP + dCMP
-
645154
Escherichia coli K-12
ADP + dCDP
-
-
-
?
ATP + dUMP
dUMP is a poor substrate
645154
Escherichia coli K-12
ADP + dUDP
-
-
-
?
ATP + UMP
-
645154
Escherichia coli
ADP + UDP
-
-
-
?
ATP + UMP
UMP is a poor substrate
645154
Escherichia coli K-12
ADP + UDP
-
-
-
?
CTP + CMP
the activity with CTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
CDP + CDP
-
-
-
?
dATP + CMP
-
645154
Escherichia coli
dADP + CDP
-
-
-
-
dATP + CMP
-
645154
Escherichia coli K-12
dADP + CDP
-
-
-
?
dCTP + CMP
the activity with dCTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dCDP + CDP
-
-
-
?
dUTP + CMP
the activity with dUTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
dUDP + CDP
-
-
-
?
GTP + CMP
-
645154
Escherichia coli
GDP + CDP
-
-
-
?
GTP + CMP
-
645154
Escherichia coli K-12
GDP + CDP
-
-
-
?
ITP + CMP
ITP is a poor substrate
645154
Escherichia coli K-12
IDP + CDP
-
-
-
?
additional information
TMP, Ant-dCMP, 5-methyl-CMP, AMP, dAMP, GMP, and dGMP are no substrates
645154
Escherichia coli K-12
?
-
-
-
-
UTP + CMP
the activity with UTP is still measurable but less than 0.05% of that with ATP
645154
Escherichia coli K-12
UDP + CDP
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
2 * 000, SDS-PAGE
Escherichia coli K-12
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
52
-
CMP kinase is half-inactivated at 52C
Escherichia coli K-12
Other publictions for EC 2.7.4.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712276
Thum
The Rv1712 locus from Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Bacteriol.
191
2884-2887
2009
-
-
1
-
-
-
-
3
-
-
3
-
-
160
-
-
1
-
-
-
-
2
6
1
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
-
2
6
1
-
-
-
3
-
-
-
-
-
1
1
-
3
3
693707
Caceres
Molecular modeling and dynamic ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
J. Mol. Model.
14
427-434
2008
-
-
1
-
-
-
-
-
-
1
-
2
-
6
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
673586
Wang
The role of Ureaplasma nucleos ...
Ureaplasma parvum
FEBS J.
274
1983-1990
2007
-
-
1
-
-
-
-
-
-
1
1
-
-
1
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676227
Alexandre
Enantioselectivity of human AM ...
Escherichia coli
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
1
7
-
-
23
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
23
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
692282
Ofiteru
Structural and functional cons ...
Escherichia coli, Escherichia coli K-12
FEBS J.
274
3363-3373
2007
-
-
2
2
14
1
-
27
-
1
1
3
-
2
-
-
1
-
-
-
1
-
8
-
-
-
1
26
-
-
-
2
-
-
-
-
-
2
2
2
14
1
-
-
-
27
-
1
1
3
-
-
-
1
-
-
1
-
8
-
-
-
1
26
-
-
-
-
-
-
-
-
23
23
675215
Lee
-
Recombinant Escherichia coli-c ...
Escherichia coli
J. Ind. Eng. Chem.
12
757-761
2006
-
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713473
Yu
Solution structure and functio ...
Streptococcus pneumoniae TIGR4
Protein Sci.
12
2613-2621
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712736
Bertrand
Sugar specificity of bacterial ...
Escherichia coli
J. Mol. Biol.
315
1099-1110
2002
-
-
1
1
3
-
-
15
-
1
-
2
-
1
-
-
1
-
-
-
-
-
5
-
-
-
1
16
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
15
-
1
-
2
-
-
-
1
-
-
-
-
5
-
-
-
1
16
-
-
-
-
-
-
-
-
12
12
645151
Li de La Sierra
Substrate-induced fit of the A ...
Escherichia coli
Biochemistry
39
15870-15878
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645163
Briozzo
Structures of Escherichia coli ...
Escherichia coli
Structure
6
1517-1527
1998
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
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-
1
-
1
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645159
Schultz
Structural and catalytic prope ...
Bacillus subtilis, Escherichia coli
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14
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