BRENDA - Enzyme Database
show all sequences of 2.7.4.25

Enantioselectivity of human AMP, dTMP and UMP-CMP kinases

Alexandre, J.A.; Roy, B.; Topalis, D.; Pochet, S.; Perigaud, C.; Deville-Bonne, D.; Nucleic Acids Res. 35, 4895-4904 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression in Escherichia coli
Escherichia coli
Crystallization (Commentary)
Crystallization (Commentary)
Organism
of the R188M mutant alone or with dCMP
Escherichia coli
Engineering
Protein Variants
Commentary
Organism
D132A
site directed mutagenesis
Escherichia coli
D132H
site directed mutagenesis
Escherichia coli
D132N
site directed mutagenesis
Escherichia coli
D132S
site directed mutagenesis
Escherichia coli
R110M
site directed mutagenesis
Escherichia coli
R188M
site directed mutagenesis
Escherichia coli
S36A
site directed mutagenesis
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
CMP
wild type
Escherichia coli
0.038
-
CMP
D132S mutant
Escherichia coli
0.055
-
dCMP
D132H mutant
Escherichia coli
0.08
-
dCMP
D132N mutant
Escherichia coli
0.09
-
dCMP
D132S mutant
Escherichia coli
0.094
-
dCMP
wild type
Escherichia coli
0.77
-
dCMP
R110M mutant
Escherichia coli
0.93
-
UMP
wild type
Escherichia coli
1
-
CMP
R110M mutant
Escherichia coli
1.3
-
CMP
D132H mutant
Escherichia coli
1.8
-
dCMP
D132A mutant
Escherichia coli
1.9
-
UMP
S36A mutant
Escherichia coli
2.5
-
CMP
S36A mutant
Escherichia coli
2.6
-
CMP
D132N mutant
Escherichia coli
2.9
-
CMP
D132A mutant
Escherichia coli
3.5
-
dCMP
S36A mutant
Escherichia coli
3.9
-
UMP
D132H mutant
Escherichia coli
4.5
-
UMP
D132N mutant
Escherichia coli
7.3
-
dCMP
R110M mutant
Escherichia coli
7.9
-
UMP
D132A mutant
Escherichia coli
8
-
UMP
D132S mutant
Escherichia coli
11.3
-
UMP
R110M mutant
Escherichia coli
20.2
-
CMP
R110M mutant
Escherichia coli
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P0A6I0
-
-
Purification (Commentary)
Purification (Commentary)
Organism
of the recombinant proteins
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + CMP
-
676227
Escherichia coli
ADP + CDP
-
-
-
?
ATP + dCMP
-
676227
Escherichia coli
ADP + dCDP
-
-
-
?
ATP + UMP
-
676227
Escherichia coli
ADP + UDP
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CMP kinase
-
Escherichia coli
cytidylate kinase
-
Escherichia coli
UMP-CMP kinase
-
Escherichia coli
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
43
-
mutant D132A
Escherichia coli
45
-
mutant R110M
Escherichia coli
47
-
mutant D132N
Escherichia coli
48
-
mutant D132S, mutant D132H
Escherichia coli
51
-
mutant R188M
Escherichia coli
52
-
wild type, mutant S36A
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
of the R188M mutant alone or with dCMP
Escherichia coli
Engineering (protein specific)
Protein Variants
Commentary
Organism
D132A
site directed mutagenesis
Escherichia coli
D132H
site directed mutagenesis
Escherichia coli
D132N
site directed mutagenesis
Escherichia coli
D132S
site directed mutagenesis
Escherichia coli
R110M
site directed mutagenesis
Escherichia coli
R188M
site directed mutagenesis
Escherichia coli
S36A
site directed mutagenesis
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.035
-
CMP
wild type
Escherichia coli
0.038
-
CMP
D132S mutant
Escherichia coli
0.055
-
dCMP
D132H mutant
Escherichia coli
0.08
-
dCMP
D132N mutant
Escherichia coli
0.09
-
dCMP
D132S mutant
Escherichia coli
0.094
-
dCMP
wild type
Escherichia coli
0.77
-
dCMP
R110M mutant
Escherichia coli
0.93
-
UMP
wild type
Escherichia coli
1
-
CMP
R110M mutant
Escherichia coli
1.3
-
CMP
D132H mutant
Escherichia coli
1.8
-
dCMP
D132A mutant
Escherichia coli
1.9
-
UMP
S36A mutant
Escherichia coli
2.5
-
CMP
S36A mutant
Escherichia coli
2.6
-
CMP
D132N mutant
Escherichia coli
2.9
-
CMP
D132A mutant
Escherichia coli
3.5
-
dCMP
S36A mutant
Escherichia coli
3.9
-
UMP
D132H mutant
Escherichia coli
4.5
-
UMP
D132N mutant
Escherichia coli
7.3
-
dCMP
R110M mutant
Escherichia coli
7.9
-
UMP
D132A mutant
Escherichia coli
8
-
UMP
D132S mutant
Escherichia coli
11.3
-
UMP
R110M mutant
Escherichia coli
20.2
-
CMP
R110M mutant
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
of the recombinant proteins
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + CMP
-
676227
Escherichia coli
ADP + CDP
-
-
-
?
ATP + dCMP
-
676227
Escherichia coli
ADP + dCDP
-
-
-
?
ATP + UMP
-
676227
Escherichia coli
ADP + UDP
-
-
-
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
43
-
mutant D132A
Escherichia coli
45
-
mutant R110M
Escherichia coli
47
-
mutant D132N
Escherichia coli
48
-
mutant D132S, mutant D132H
Escherichia coli
51
-
mutant R188M
Escherichia coli
52
-
wild type, mutant S36A
Escherichia coli
Other publictions for EC
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
712276
Thum
The Rv1712 locus from Mycobact ...
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
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2884-2887
2009
-
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1
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1
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-
-
-
2
6
1
2
-
-
-
3
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
3
-
-
-
-
1
-
-
-
2
6
1
-
-
-
3
-
-
-
-
-
1
1
-
3
3
693707
Caceres
Molecular modeling and dynamic ...
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1
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-
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-
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2
-
6
-
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-
-
-
-
-
-
6
1
9
-
-
-
-
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-
-
1
-
-
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-
1
2
-
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1
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2
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-
-
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-
6
1
-
-
-
-
-
-
-
-
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-
-
-
-
-
673586
Wang
The role of Ureaplasma nucleos ...
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1983-1990
2007
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1
-
-
-
-
-
4
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
676227
Alexandre
Enantioselectivity of human AM ...
Escherichia coli
Nucleic Acids Res.
35
4895-4904
2007
-
-
1
1
7
-
-
23
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
3
-
-
6
-
-
-
-
-
-
-
-
-
-
1
-
1
7
-
-
-
-
23
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
692282
Ofiteru
Structural and functional cons ...
Escherichia coli, Escherichia coli K-12
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-
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2
2
14
1
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27
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1
1
3
-
2
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1
-
-
-
1
-
8
-
2
-
-
1
26
-
-
-
2
-
-
-
-
-
2
2
2
14
1
-
-
-
27
-
1
1
3
-
-
-
1
-
-
1
-
8
-
-
-
1
26
-
-
-
-
-
-
-
-
23
23
675215
Lee
-
Recombinant Escherichia coli-c ...
Escherichia coli
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12
757-761
2006
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1
1
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1
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-
-
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1
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1
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1
1
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1
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1
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-
-
-
-
-
-
-
-
713473
Yu
Solution structure and functio ...
Streptococcus pneumoniae TIGR4
Protein Sci.
12
2613-2621
2003
-
-
-
-
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-
-
-
-
-
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5
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-
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-
-
-
1
-
2
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-
-
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1
-
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-
1
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
1
1
-
-
-
712736
Bertrand
Sugar specificity of bacterial ...
Escherichia coli
J. Mol. Biol.
315
1099-1110
2002
-
-
1
1
3
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15
-
1
-
2
-
1
-
-
1
-
-
-
-
-
5
-
2
-
-
1
16
-
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
15
-
1
-
2
-
-
-
1
-
-
-
-
5
-
-
-
1
16
-
-
-
-
-
-
-
-
12
12
645151
Li de La Sierra
Substrate-induced fit of the A ...
Escherichia coli
Biochemistry
39
15870-15878
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
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-
-
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-
-
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-
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-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645163
Briozzo
Structures of Escherichia coli ...
Escherichia coli
Structure
6
1517-1527
1998
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
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1
-
1
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-
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-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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645159
Schultz
Structural and catalytic prope ...
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340
144-153
1997
-
-
1
-
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-
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10
-
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2
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1
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-
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14
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2
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6
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1
-
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-
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-
1
1
-
-
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-
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10
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-
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-
-
-
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1
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14
-
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6
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645154
Bucurenci
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2856-2862
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-
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1
16
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2
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-
-
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20
1
1
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
2
1
-
-
1
-
16
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-
1
-
-
-
-
2
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-
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20
1
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1
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-
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-
712269
Fricke
The cmk gene encoding cytidine ...
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-
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3
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2
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-
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-
-
1
-
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-
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-
1
-
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-
-
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-
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-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
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-
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2
2
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712266
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1
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3
-
3
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4
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1
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1
1
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3
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3
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