EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.4.23.18 | 55 |
- |
10 min, in 50 mM sodium acetate buffer, pH 5 |
30569, 30570, 30575 |
3.4.23.18 | 60 |
- |
20 min, at pH 2.8, complete inactivation |
30560 |
3.4.23.18 | 55 |
- |
20 min, complete inactivation at pH 5.5, Ca2+ does not protect |
30564 |
3.4.23.18 | 20 |
45 |
after incubation at 20°C for 1 h, the enzyme with propeptide retains 100% activity. 38.1°C is the melting temperature of the catalytically active enzyme with propeptide. When the temperature is over 45°C, the relative enzyme activities of the enzyme with propeptide decreases to below 40% |
755227 |
3.4.23.18 | 40 |
- |
below, stable |
30560 |
3.4.23.18 | 55 |
- |
isozyme A1 loses 10% of its original activity in contrast to almost complete loss of activity of isozyme A2 |
30569 |
3.4.23.18 | 55 |
- |
isozyme F1 retains 70%, F2 85% of original activity |
30570 |
3.4.23.18 | 55 |
- |
isozyme M1 retains more than 95% of its original activity, M2 is completely inactivated |
30575 |
3.4.23.18 | 20 |
25 |
pH 2.7, 16 h stable |
30564 |
3.4.23.18 | 30 |
- |
pH 7, drastic decrease of activity probably due to autolytic activity |
30564 |