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Literature summary for 3.4.23.18 extracted from
- Presence and partial characterization of internal acid protease of Aspergillus oryzae (1978), Appl. Environ. Microbiol., 36, 237-242.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | not | Aspergillus oryzae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | soybean trypsin inhibitor; Triton X-100 | Aspergillus oryzae | |
| SDS | - |
Aspergillus oryzae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | 2 forms: extracellular and cytoplasmic, the cytoplasmic isozymes are immunologically related to extracellular isozyme A2 | Aspergillus oryzae | 5737 | - |
| extracellular | 2 forms: extracellular and cytoplasmic | Aspergillus oryzae | - |
- |
| soluble | - |
Aspergillus oryzae | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
Aspergillus oryzae, enzyme form F2, gel filtration | Aspergillus oryzae |
| 60000 | - |
Aspergillus oryzae, enzyme form F1, gel filtration | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
- |
- |
| Aspergillus oryzae 365-U-64-1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cytoplasmic enzyme: partial | Aspergillus oryzae |
| several isoforms (cytoplasmic F1 and F2) | Aspergillus oryzae |
| several isoforms (extracellular A1 and A2) | Aspergillus oryzae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | extracellular enzyme | Aspergillus oryzae | - |
| commercial preparation | Taka-diastase, crude Aspergillus oryzae powder preparation | Aspergillus oryzae | - |
| mycelium | cytoplasmic enzyme | Aspergillus oryzae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Cytochrome c + H2O | - |
Aspergillus oryzae | ? | - |
? | |
| Cytochrome c + H2O | - |
Aspergillus oryzae 365-U-64-1 | ? | - |
? | |
| Hemoglobin + H2O | urea-denatured hemoglobin | Aspergillus oryzae | ? | - |
? | |
| Hemoglobin + H2O | urea-denatured hemoglobin | Aspergillus oryzae 365-U-64-1 | ? | - |
? | |
| Trypsinogen + H2O | bovine (pancreatic) | Aspergillus oryzae | ? | - |
? | |
| Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae | ? | - |
? | |
| Trypsinogen + H2O | bovine (pancreatic) | Aspergillus oryzae 365-U-64-1 | ? | - |
? | |
| Trypsinogen + H2O | bovine (trypsinogen) | Aspergillus oryzae 365-U-64-1 | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
10 min, in 50 mM sodium acetate buffer, pH 5 | Aspergillus oryzae |
| 55 | - |
isozyme F1 retains 70%, F2 85% of original activity | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
cytochrome c | Aspergillus oryzae |
| 4.2 | - |
urea-denatured hemoglobin | Aspergillus oryzae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.3 | 4.3 | about half-maximal activity at pH 2.3 and 4.3, cytochrome c | Aspergillus oryzae |
| 3.5 | 6.5 | about half-maximal activity at pH 3.5 and 6.5, urea-denatured hemoglobin | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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