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Literature summary for 3.4.23.18 extracted from

  • Ichishima, E.
    Purification and mode of assay for acid proteinase of Aspergillus saitoi (1970), Methods Enzymol., 19, 397-406.
No PubMed abstract available
Search for more literature for 3.4.23.18

Activating Compound

Activating Compound Comment Organism Structure
additional information no activator required Aspergillus phoenicis

General Stability

General Stability Organism
Photooxidation at pH 5.5 inactivates Aspergillus phoenicis
Stable to low urea concentrations, but 5 M, at an apparent pH of 5.5 inactivates 46% after 30 min and 59% after 60 min incubation Aspergillus phoenicis

Inhibitors

Inhibitors Comment Organism Structure
N-Acetylimidazole
-
 Aspergillus phoenicis
N-bromosuccinimide
-
 Aspergillus phoenicis
SDS
-
 Aspergillus phoenicis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0125
-
 Trypsinogen 30°C, pH 3.5 Aspergillus phoenicis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus phoenicis
-
-
soluble
-
 Aspergillus phoenicis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion required Aspergillus phoenicis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 amino acid composition Aspergillus phoenicis
34200
-
 Aspergillus saitoi, sedimentation equilibrium, meniscus depletion methods Aspergillus phoenicis
34500
-
 Aspergillus saitoi, gel filtration Aspergillus phoenicis

Organism

Organism UniProt Comment Textmining
Aspergillus phoenicis
-
-
-
Aspergillus phoenicis R-3813
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from koji culture and submerged cell culture Aspergillus phoenicis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture surface culture Aspergillus phoenicis
-
cell culture submerged culture Aspergillus phoenicis
-
culture filtrate
-
 Aspergillus phoenicis
-
Koji culture
-
 Aspergillus phoenicis
-

Storage Stability

Storage Stability Organism
20-25°C, pH 2.7, 16 h Aspergillus phoenicis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Chymotrypsinogen + H2O
-
 Aspergillus phoenicis Pi-Chymotrypsin
-
 ?
Chymotrypsinogen + H2O
-
 Aspergillus phoenicis R-3813 Pi-Chymotrypsin
-
 ?
additional information no milk clotting activity Aspergillus phoenicis ?
-
 ?
additional information no milk clotting activity Aspergillus phoenicis R-3813 ?
-
 ?
Trypsinogen + H2O cleaves Lys-Ile and liberates hexapeptide Aspergillus phoenicis ?
-
 ?
Trypsinogen + H2O cleaves Lys-Ile and liberates hexapeptide Aspergillus phoenicis R-3813 ?
-
 ?

Synonyms

Synonyms Comment Organism
Aspergillopeptidase A
-
 Aspergillus phoenicis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 25 pH 2.7, 16 h stable Aspergillus phoenicis
30
-
 pH 7, drastic decrease of activity probably due to autolytic activity Aspergillus phoenicis
55
-
 20 min, complete inactivation at pH 5.5, Ca2+ does not protect Aspergillus phoenicis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 3.65 Aspergillus phoenicis
3.5
-
-
 Aspergillus phoenicis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
 most stable at Aspergillus phoenicis
6 7 above pH 7 denaturation and inactivation Aspergillus phoenicis