BRENDA - Enzyme Database show
show all sequences of 3.4.23.18

Purification and characterization of the two molecular forms of membrane acid protease from Aspergillus oryzae

Tsujita, Y.; Endo, A.; Eur. J. Biochem. 84, 347-353 (1978)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
cholesterol
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
Diglycerides
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
monoglycerides
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
additional information
no activation of membrane-bound enzyme by triglycerides or 1,3-dipalmitin
Aspergillus oryzae
Phospholipids
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
Triton X-100
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
diisopropyl phosphofluoridate; monoiodoacetate, 1,10-phenanthroline, 6-aminohexanoate; PCMB
Aspergillus oryzae
N-bromosuccinimide
not
Aspergillus oryzae
SDS
3.3 mM
Aspergillus oryzae
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aspergillus oryzae
16020
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
Aspergillus oryzae, enzyme form M2, gel filtration
Aspergillus oryzae
230000
-
Aspergillus oryzae, Triton X-100 treated enzyme, gel filtration
Aspergillus oryzae
2000000
-
Aspergillus oryzae, enzyme form M1, gel filtration
Aspergillus oryzae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus oryzae
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
80.5% (enzyme form M1), 52.5% (enzyme form M2)
Aspergillus oryzae
Purification (Commentary)
Commentary
Organism
several isoforms (membrane-bound M1 and M2)
Aspergillus oryzae
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus oryzae
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
143
-
enzyme form M1, in the presence of Triton X-100
Aspergillus oryzae
867
-
enzyme form M2, in the presence of Triton X-100
Aspergillus oryzae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Cytochrome c + H2O
-
30575
Aspergillus oryzae
?
-
-
-
-
Hemoglobin + H2O
urea-denatured hemoglobin
30575
Aspergillus oryzae
?
-
-
-
-
Trypsinogen + H2O
-
30575
Aspergillus oryzae
?
-
-
-
-
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
10 min, in 50 mM sodium acetate buffer, pH 5; isozyme M1 retains more than 95% of its original activity, M2 is completely inactivated
Aspergillus oryzae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2
-
cytochrome c
Aspergillus oryzae
4.5
-
urea-denatured hemoglobin
Aspergillus oryzae
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
3
6
stable in this range, complete inactivation below 2 and above 7
Aspergillus oryzae
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
cholesterol
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
Diglycerides
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
monoglycerides
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
additional information
no activation of membrane-bound enzyme by triglycerides or 1,3-dipalmitin
Aspergillus oryzae
Phospholipids
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
Triton X-100
activation, acetone-washed membrane-bound enzyme
Aspergillus oryzae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
diisopropyl phosphofluoridate; monoiodoacetate, 1,10-phenanthroline, 6-aminohexanoate; PCMB
Aspergillus oryzae
N-bromosuccinimide
not
Aspergillus oryzae
SDS
3.3 mM
Aspergillus oryzae
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Aspergillus oryzae
16020
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
100000
-
Aspergillus oryzae, enzyme form M2, gel filtration
Aspergillus oryzae
230000
-
Aspergillus oryzae, Triton X-100 treated enzyme, gel filtration
Aspergillus oryzae
2000000
-
Aspergillus oryzae, enzyme form M1, gel filtration
Aspergillus oryzae
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
80.5% (enzyme form M1), 52.5% (enzyme form M2)
Aspergillus oryzae
Purification (Commentary) (protein specific)
Commentary
Organism
several isoforms (membrane-bound M1 and M2)
Aspergillus oryzae
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus oryzae
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
143
-
enzyme form M1, in the presence of Triton X-100
Aspergillus oryzae
867
-
enzyme form M2, in the presence of Triton X-100
Aspergillus oryzae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Cytochrome c + H2O
-
30575
Aspergillus oryzae
?
-
-
-
-
Hemoglobin + H2O
urea-denatured hemoglobin
30575
Aspergillus oryzae
?
-
-
-
-
Trypsinogen + H2O
-
30575
Aspergillus oryzae
?
-
-
-
-
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
55
-
10 min, in 50 mM sodium acetate buffer, pH 5; isozyme M1 retains more than 95% of its original activity, M2 is completely inactivated
Aspergillus oryzae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2
-
cytochrome c
Aspergillus oryzae
4.5
-
urea-denatured hemoglobin
Aspergillus oryzae
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
3
6
stable in this range, complete inactivation below 2 and above 7
Aspergillus oryzae
Other publictions for EC 3.4.23.18
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
680281
Landbo
Protease-assisted clarificatio ...
Aspergillus niger
J. Agric. Food Chem.
54
6554-6563
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
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-
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-
-
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-
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668006
Zhu
Analysis of secreted proteins ...
Aspergillus oryzae, Aspergillus oryzae RIB 40
Biosci. Biotechnol. Biochem.
68
2607-2612
2004
-
-
-
-
-
-
1
-
1
-
2
-
-
4
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
-
2
-
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-
1
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668812
Ichishima
-
Aspergillopepsin I ...
Aspergillus awamori, Aspergillus foetidus, Aspergillus fumigatus, Aspergillus kawachii, Aspergillus niger, Aspergillus oryzae, Aspergillus phoenicis, Aspergillus phoenicis R-3813, Aspergillus sojae
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
92-99
2004
-
5
5
1
1
-
1
-
8
-
2
9
-
10
-
1
1
1
1
9
-
-
26
6
-
-
-
-
4
1
1
-
-
-
-
-
5
5
-
1
1
-
-
1
-
-
8
-
2
9
-
-
1
1
1
9
-
-
26
6
-
-
-
-
4
1
1
-
-
-
-
-
-
-
652904
Kamitori
Crystal structures of Aspergil ...
Aspergillus oryzae
J. Mol. Biol.
326
1503-1511
2003
-
-
-
1
-
-
1
-
-
-
1
-
-
2
-
-
1
-
-
-
-
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-
-
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1
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1
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-
1
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649170
Cho
Structure of aspergillopepsin ...
Aspergillus phoenicis
Acta Crystallogr. Sect. D
57
948-956
2001
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
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1
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1
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-
-
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-
-
-
-
-
-
-
-
30556
Lu
Molecular cloning of a cDNA fo ...
Aspergillus awamori, Aspergillus niger, Aspergillus phoenicis
Biosci. Biotechnol. Biochem.
59
954-955
1995
-
-
-
-
-
-
3
-
6
-
3
-
-
5
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
6
-
3
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
30558
Tello-Solis
Effect of irreversibility on t ...
Aspergillus phoenicis
Biochem. J.
311
969-974
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
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-
1
-
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-
1
1
-
-
-
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-
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-
-
-
-
-
-
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-
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-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
30557
Takeuchi
-
Fluorogenic substrate of Asper ...
Aspergillus phoenicis
Agric. Biol. Chem.
52
1279-1280
1988
-
-
-
-
-
-
1
-
2
-
-
-
-
1
-
-
1
-
-
1
-
-
3
-
1
-
-
-
1
-
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-
-
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-
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-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
1
-
1
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
30559
Majima
-
Comparative study on the speci ...
Aspergillus oryzae, Aspergillus phoenicis, Aspergillus sojae
Agric. Biol. Chem.
52
787-793
1988
-
-
-
-
-
-
-
-
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-
-
3
-
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3
-
3
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-
-
3
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
30560
Yagi
Purification and characterizat ...
Aspergillus kawachii
Agric. Biol. Chem.
50
1029-1033
1986
-
-
-
-
-
-
3
-
2
-
2
-
-
1
-
-
1
-
-
2
1
-
1
1
2
-
2
-
2
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-
3
-
-
2
-
2
-
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1
-
2
1
-
1
1
2
-
2
-
2
-
-
-
-
-
-
-
-
-
30574
Krishnan
Purification of an acid protea ...
Aspergillus niger
J. Chromatogr.
329
165-170
1985
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-
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2
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1
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1
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1
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1
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30561
Bhumibhamon
-
Precipitation and characterist ...
Aspergillus phoenicis
Thai J. Agric. Sci.
15
157-172
1982
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-
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2
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1
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1
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1
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1
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1
1
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2
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1
-
1
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-
1
-
1
1
-
-
-
-
-
-
-
-
30562
Panneerselvam
Physico-chemical properties of ...
Aspergillus fumigatus
Ital. J. Biochem.
30
63-74
1981
-
-
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-
1
-
1
-
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1
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1
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1
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1
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1
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1
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1
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1
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1
-
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-
1
-
1
-
-
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1
-
1
-
-
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-
-
-
-
30563
Bhumibhamon
-
Some characreristics of the ac ...
Aspergillus awamori
Thai J. Agric. Sci.
12
27-33
1979
-
-
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1
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1
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1
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1
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1
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1
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1
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1
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-
-
-
30570
Tsujita
Presence and partial character ...
Aspergillus oryzae, Aspergillus oryzae 365-U-64-1
Appl. Environ. Microbiol.
36
237-242
1978
1
-
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2
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3
-
2
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3
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1
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2
1
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8
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1
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2
2
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1
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2
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3
-
2
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1
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2
1
-
8
-
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1
-
2
2
-
-
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-
-
-
-
-
30575
Tsujita
Purification and characterizat ...
Aspergillus oryzae
Eur. J. Biochem.
84
347-353
1978
6
-
-
-
-
-
3
-
1
-
3
-
-
3
-
1
1
-
-
1
2
-
3
-
-
-
1
-
2
-
1
-
-
-
-
6
-
-
-
-
-
-
-
3
-
-
1
-
3
-
-
-
1
1
-
1
2
-
3
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
30571
Tanaka
Purification of an acid protei ...
Aspergillus phoenicis
Biochim. Biophys. Acta
485
406-416
1977
-
-
-
-
-
-
-
-
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1
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5
-
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5
-
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-
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-
30568
Takahashi
The structure and function of ...
Aspergillus niger, Aspergillus phoenicis
J. Biochem.
80
497-506
1976
-
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4
-
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2
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2
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4
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2
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-
30569
Tsujita
Purification and characterizat ...
Aspergillus oryzae
Biochim. Biophys. Acta
445
194-204
1976
-
-
-
1
-
-
3
-
-
1
3
-
-
3
-
1
1
-
-
1
-
1
5
-
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1
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3
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1
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3
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1
3
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1
1
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1
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1
5
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1
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3
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30572
Chang
The structure and function of ...
Aspergillus niger
J. Biochem.
80
975-981
1976
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5
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5
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5
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30573
Iio
Specificity of acid proteinase ...
Aspergillus niger
Biochim. Biophys. Acta
429
912-924
1976
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2
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1
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1
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1
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2
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-
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-
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-
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1
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1
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30567
Davidson
Aspergillus oryzae acid protei ...
Aspergillus oryzae
Biochem. J.
147
45-53
1975
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2
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2
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1
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1
1
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6
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1
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2
1
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2
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2
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1
1
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6
-
1
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2
1
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30566
Morihara
Comparative specificity of mic ...
Aspergillus niger, Aspergillus niger B, Aspergillus phoenicis
Arch. Biochem. Biophys.
157
561-572
1973
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7
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4
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22
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2
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8
1
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7
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22
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2
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8
1
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30565
Kovaleva
The site of diazoacetyl inhibi ...
Aspergillus awamori
Biochem. Biophys. Res. Commun.
49
1075-1081
1972
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2
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1
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1
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1
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2
1
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2
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1
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1
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2
1
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30564
Ichishima
-
Purification and mode of assay ...
Aspergillus phoenicis, Aspergillus phoenicis R-3813
Methods Enzymol.
19
397-406
1970
1
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2
3
1
2
1
3
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3
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1
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3
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6
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3
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2
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2
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1
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2
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3
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1
2
1
3
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1
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3
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1
6
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3
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2
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2
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