EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
5.3.2.6 | 0.36 |
- |
2-hydroxy-2,4-pentadienoate |
pH 7.3, 23°C, wild-type enzyme |
718830 |
5.3.2.6 | 0.45 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to compound 5 |
721792 |
5.3.2.6 | 0.45 |
- |
2-hydroxymuconate |
mutant enzyme R39A, in 10 mM potassium phosphate buffer, pH 7.3, at 22°C |
726964 |
5.3.2.6 | 0.9 |
- |
2-hydroxy-2,4-pentadienoate |
pH 7.3, 23°C, mutant R11A |
718830 |
5.3.2.6 | 1.1 |
- |
2-hydroxy-2,4-pentadienoate |
pH 7.3, 23°C |
721792 |
5.3.2.6 | 1.2 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate |
721792 |
5.3.2.6 | 3.2 |
- |
phenylenolpyruvate |
pH 7.3, 24°C, recombinant hh4-OT mutant betaP1A |
718874 |
5.3.2.6 | 4.2 |
- |
phenylenolpyruvate |
pH 7.3, 24°C, recombinant hh4-OT mutant alphaR12A |
718874 |
5.3.2.6 | 4.4 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to compound 5 |
721792 |
5.3.2.6 | 5.4 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate |
721792 |