Cloned (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
- |
Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
crystals are grown from 20% dioxane and 50% 2-methyl-2,4-pentanediol, pH 6.0, vapor diffusion method | Archaeoglobus fulgidus |
crystals are grown from 28% polyethylene glycol 400, 200 mM CaCl2, 0.1 M HEPES buffer (pH 7.5), vapor diffusion method | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
phenylenolpyruvate | pH 7.3, 23°C | Archaeoglobus fulgidus | |
0.09 | - |
2-hydroxy-2,4-pentadienoate | pH 7.3, 23°C | Archaeoglobus fulgidus | |
0.11 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate | Helicobacter pylori | |
0.11 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to compound 5 | Archaeoglobus fulgidus | |
0.135 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to compound 5 | Helicobacter pylori | |
0.17 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate | Archaeoglobus fulgidus | |
0.19 | - |
2-hydroxy-2,4-pentadienoate | pH 7.3, 23°C | Helicobacter pylori | |
0.23 | - |
phenylenolpyruvate | pH 7.3, 23°C | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29588 | - |
- |
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | - |
Archaeoglobus fulgidus | (2E)-5-oxohex-2-enedioate | - |
? | |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | - |
Helicobacter pylori | (2E)-5-oxohex-2-enedioic acid | - |
? | |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | - |
Helicobacter pylori | (3E)-2-oxohex-3-enedioate | - |
? | |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | - |
Archaeoglobus fulgidus | (3E)-2-oxohex-3-enedioate | - |
? | |
2-hydroxy-2,4-pentadienoate | - |
Helicobacter pylori | 2-oxo-4-pentenoate | - |
? | |
2-hydroxy-2,4-pentadienoate | - |
Archaeoglobus fulgidus | 2-oxo-4-pentenoate | - |
? | |
phenylenolpyruvate | - |
Helicobacter pylori | phenylpyruvate | - |
? | |
phenylenolpyruvate | - |
Archaeoglobus fulgidus | phenylpyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | the betaalphabeta fold is conserved in the monomer | Helicobacter pylori |
hexamer | the betaalphabeta fold is conserved in the monomer | Archaeoglobus fulgidus |
Synonyms | Comment | Organism |
---|---|---|
4-oxalocrotonate tautomerase | - |
Helicobacter pylori |
4-oxalocrotonate tautomerase | - |
Archaeoglobus fulgidus |
AfDmpI | - |
Archaeoglobus fulgidus |
HpDmpI | - |
Helicobacter pylori |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Helicobacter pylori |
23 | - |
assay at | Archaeoglobus fulgidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to compound 5 | Archaeoglobus fulgidus | |
0.1 | - |
2-hydroxy-2,4-pentadienoate | pH 7.3, 23°C | Archaeoglobus fulgidus | |
0.2 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate | Archaeoglobus fulgidus | |
0.6 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate | Helicobacter pylori | |
0.6 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to compound 5 | Helicobacter pylori | |
2.4 | - |
phenylenolpyruvate | pH 7.3, 23°C | Archaeoglobus fulgidus | |
2.5 | - |
2-hydroxy-2,4-pentadienoate | pH 7.3, 23°C | Helicobacter pylori | |
2.6 | - |
phenylenolpyruvate | pH 7.3, 23°C | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Helicobacter pylori |
7.3 | - |
assay at | Archaeoglobus fulgidus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to compound 5 | Archaeoglobus fulgidus | |
1.1 | - |
2-hydroxy-2,4-pentadienoate | pH 7.3, 23°C | Archaeoglobus fulgidus | |
1.2 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate | Archaeoglobus fulgidus | |
4.4 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to compound 5 | Helicobacter pylori | |
5.4 | - |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate | pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate | Helicobacter pylori | |
11 | - |
phenylenolpyruvate | pH 7.3, 23°C | Helicobacter pylori | |
13 | - |
2-hydroxy-2,4-pentadienoate | pH 7.3, 23°C | Helicobacter pylori | |
27 | - |
phenylenolpyruvate | pH 7.3, 23°C | Archaeoglobus fulgidus |