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Literature summary for 5.3.2.6 extracted from

  • Almrud, J.J.; Dasgupta, R.; Czerwinski, R.M.; Kern, A.D.; Hackert, M.L.; Whitman, C.P.
    Kinetic and structural characterization of DmpI from Helicobacter pylori and Archaeoglobus fulgidus, two 4-oxalocrotonate tautomerase family members (2010), Bioorg. Chem., 38, 252-259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
-
Helicobacter pylori
-
Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals are grown from 20% dioxane and 50% 2-methyl-2,4-pentanediol, pH 6.0, vapor diffusion method Archaeoglobus fulgidus
crystals are grown from 28% polyethylene glycol 400, 200 mM CaCl2, 0.1 M HEPES buffer (pH 7.5), vapor diffusion method Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
phenylenolpyruvate pH 7.3, 23°C Archaeoglobus fulgidus
0.09
-
2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Archaeoglobus fulgidus
0.11
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Helicobacter pylori
0.11
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Archaeoglobus fulgidus
0.135
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Helicobacter pylori
0.17
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Archaeoglobus fulgidus
0.19
-
2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Helicobacter pylori
0.23
-
phenylenolpyruvate pH 7.3, 23°C Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O29588
-
-
Helicobacter pylori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Helicobacter pylori
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
Archaeoglobus fulgidus (2E)-5-oxohex-2-enedioate
-
?
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
Helicobacter pylori (2E)-5-oxohex-2-enedioic acid
-
?
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
Helicobacter pylori (3E)-2-oxohex-3-enedioate
-
?
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate
-
Archaeoglobus fulgidus (3E)-2-oxohex-3-enedioate
-
?
2-hydroxy-2,4-pentadienoate
-
Helicobacter pylori 2-oxo-4-pentenoate
-
?
2-hydroxy-2,4-pentadienoate
-
Archaeoglobus fulgidus 2-oxo-4-pentenoate
-
?
phenylenolpyruvate
-
Helicobacter pylori phenylpyruvate
-
?
phenylenolpyruvate
-
Archaeoglobus fulgidus phenylpyruvate
-
?

Subunits

Subunits Comment Organism
hexamer the beta–alpha–beta fold is conserved in the monomer Helicobacter pylori
hexamer the beta–alpha–beta fold is conserved in the monomer Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
4-oxalocrotonate tautomerase
-
Helicobacter pylori
4-oxalocrotonate tautomerase
-
Archaeoglobus fulgidus
AfDmpI
-
Archaeoglobus fulgidus
HpDmpI
-
Helicobacter pylori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
assay at Helicobacter pylori
23
-
assay at Archaeoglobus fulgidus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.05
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Archaeoglobus fulgidus
0.1
-
2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Archaeoglobus fulgidus
0.2
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Archaeoglobus fulgidus
0.6
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Helicobacter pylori
0.6
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Helicobacter pylori
2.4
-
phenylenolpyruvate pH 7.3, 23°C Archaeoglobus fulgidus
2.5
-
2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Helicobacter pylori
2.6
-
phenylenolpyruvate pH 7.3, 23°C Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Helicobacter pylori
7.3
-
assay at Archaeoglobus fulgidus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.45
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Archaeoglobus fulgidus
1.1
-
2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Archaeoglobus fulgidus
1.2
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Archaeoglobus fulgidus
4.4
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to compound 5 Helicobacter pylori
5.4
-
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate pH 7.3, 23°C, conversion to (3E)-2-oxohex-3-enedioate Helicobacter pylori
11
-
phenylenolpyruvate pH 7.3, 23°C Helicobacter pylori
13
-
2-hydroxy-2,4-pentadienoate pH 7.3, 23°C Helicobacter pylori
27
-
phenylenolpyruvate pH 7.3, 23°C Archaeoglobus fulgidus