EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    5.3.2.6 | 26600 |
- |
(2Z,4E)-2-hydroxyhexa-2,4-dienedioate |
at pH 7.3 and 22°C |
747782 |
| 5.3.2.6 | 32000 |
- |
2-oxo-4(E)-hexenedioate |
D-4OT enantiomer, pH 7.3, 30°C |
719636 |
| 5.3.2.6 | 1000 |
- |
5-(methyl)-2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 33000 |
- |
2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase I, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 16 |
- |
5-(methyl)-2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 310 |
- |
2-hydroxymuconate |
isoform 4-oxalocrotonate tautomerase II, in 10 mM potassium phosphate buffer, pH 7.3, at 24°C |
726816 |
| 5.3.2.6 | 29000 |
- |
2-oxo-4(E)-hexenedioate |
L-4OT enantiomer, pH 7.3, 30°C |
719636 |
| 5.3.2.6 | 520 |
- |
2-hydroxymuconate |
mutant 4-OT I52E, pH 7.3, 23°C |
718844 |
| 5.3.2.6 | 3600 |
- |
2-hydroxymuconate |
mutant 4-OT L8R, pH 7.3, 23°C |
718844 |
| 5.3.2.6 | 11 |
- |
2-hydroxymuconate |
mutant 4-OT L8R/I52E, pH 7.3, 23°C |
718844 |
