EC Number   |
General Information |
Reference  |
|---|
   1.13.11.68 | physiological function |
coexpression of the enzyme, CCD7, and all-trans-10'-apo-beta-carotenal 13,14-cleaving dioxygenase CCD8, EC 1.13.11.70, in Escherichia coli results in production of 13-apo-beta-carotenone. The sequential cleavages of beta-carotene by CCD7 and CCD8 are likely the initial steps in the synthesis of a carotenoid-derived signaling molecule that is necessary for the regulation lateral branching |
719823 |
| 1.13.11.68 | physiological function |
loss-of-function mutants exhibit a significant decrease in petiole length and are highly branched. The axillary buds, which are typically delayed in growth in wild-type plants, grow out to produce leaves and inflorescences. The mutant plant have smaller rosette diameters due to a decrease in the lengths of petioles and leaf blades compared with wild-type plants. The phenotypes contribute to the bushy appearance of the mutants. The double mutant, additionally lacking 10'-apo-beta-carotenal 13,14-cleaving dioxygenase activity, EC 1.13.11.70, is phenotypically indistinguishable from either single mutant, indicating an interaction consistent with both genes functioning in the same pathway. Both classes of plants show a slight increase in inflorescence number compared with wild type |
720702 |
| 1.13.11.68 | malfunction |
tomato plants expressing a SlCCD7 antisense construct display greatly increased branching. A metabolomic screen is conducted. With the exception of a reduction of stem amino acid content in the transgenic lines, no major changes are observed. Targeted analysis of the same plants reveal significantly decreased levels of strigolactone. There are no significant changes in root carotenoids, indicating that relatively little substrate is required to produce the bioactive strigolactones. The germination rate of Orobanche ramosa seeds is reduced by up to 90% on application of extract from the SlCCD7 antisense lines, compared with the wild type. Upon mycorrhizal colonization, C13 cyclohexenone and C14 mycorradicin apocarotenoid levels are greatly reduced in the roots of the antisense lines, implicating SlCCD7 in their biosynthesis |
728478 |
| 1.13.11.68 | physiological function |
two carotenoid cleavage dioxygenases, CCD7 and CCD8, are involved in strigolactones biosynthesis. Involvement of the branching enzymes CCD7 and CCD8 in the control of bud sprouting and apical dominance. CsCCD7 and CsCCD8 expression show some overlapping, although they are not identical |
744603 |
| 1.13.11.68 | metabolism |
biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene, strigolactone biosynthesis pathway from all-trans-beta-carotene to ent-2'-epi-5-deoxystrigol |
744876 |
| 1.13.11.68 | more |
the biochemical basis of the shoot branching phenotype is not due to inhibition of enzyme CCD7, but of enzyme CCD8, EC 1.13.11.70 |
744876 |
| 1.13.11.68 | physiological function |
biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene |
744876 |
| 1.13.11.68 | physiological function |
strigolactones are phytohormones synthesized from carotenoids via a stereospecific pathway involving the carotenoid cleavage dioxygenases 7 (CCD7) and 8. CCD7 cleaves 9-cis-beta-carotene to form a supposedly 9-cis-configured beta-apo-10'-carotenal |
744898 |
| 1.13.11.68 | evolution |
occurrence of duplication in CCD4 genes that evolved into two new genes CCD7 and CCD8, EC 1.13.11.70. The site-specific profile and coefficient of type-I functional divergences reveals critical amino acid residues, leading to subgroup-specific functional evolution after their phylogenetic diversification |
745435 |
| 1.13.11.68 | more |
in silico analysis, structure homology modeling, molecular modeling, dynamic simulation and structure comparisons of Arabidopsis thaliana carotenoid cleavage dioxygenases, overview |
745435 |
