BRENDA - Enzyme Database
show all sequences of 1.13.11.68

Biochemical characterization and selective inhibition of beta-carotene cis-trans isomerase D27 and carotenoid cleavage dioxygenase CCD8 on the strigolactone biosynthetic pathway

Harrison, P.J.; Newgas, S.A.; Descombes, F.; Shepherd, S.A.; Thompson, A.J.; Bugg, T.D.; FEBS J. 282, 3986-4000 (2015)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene ccd7, recombinant expression of GST-tagged AtCCD7 in Escherichia coli
Arabidopsis thaliana
Inhibitors
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by hydroxamic acids
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
9-cis-beta-carotene + O2
Arabidopsis thaliana
-
9-cis-10'-apo-beta-carotenal + beta-ionone
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q7XJM2
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant GST-tagged AtCCD7 from Escherichia coli by glutathione affinity chromatographyy
Arabidopsis thaliana
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
9-cis-beta-carotene + O2
-
744876
Arabidopsis thaliana
9-cis-10'-apo-beta-carotenal + beta-ionone
-
-
-
?
9-cis-beta-carotene + O2
AtCCD7 preferentially cleaves 9-cis-beta-carotene
744876
Arabidopsis thaliana
9-cis-10'-apo-beta-carotenal + beta-ionone
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
carotenoid cleavage dioxygenase
-
Arabidopsis thaliana
CCD7
-
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ccd7, recombinant expression of GST-tagged AtCCD7 in Escherichia coli
Arabidopsis thaliana
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
additional information
no inhibition by hydroxamic acids
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
9-cis-beta-carotene + O2
Arabidopsis thaliana
-
9-cis-10'-apo-beta-carotenal + beta-ionone
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged AtCCD7 from Escherichia coli by glutathione affinity chromatographyy
Arabidopsis thaliana
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
9-cis-beta-carotene + O2
-
744876
Arabidopsis thaliana
9-cis-10'-apo-beta-carotenal + beta-ionone
-
-
-
?
9-cis-beta-carotene + O2
AtCCD7 preferentially cleaves 9-cis-beta-carotene
744876
Arabidopsis thaliana
9-cis-10'-apo-beta-carotenal + beta-ionone
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
metabolism
biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene, strigolactone biosynthesis pathway from all-trans-beta-carotene to ent-2'-epi-5-deoxystrigol
Arabidopsis thaliana
additional information
the biochemical basis of the shoot branching phenotype is not due to inhibition of enzyme CCD7, but of enzyme CCD8, EC 1.13.11.70
Arabidopsis thaliana
physiological function
biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
metabolism
biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene, strigolactone biosynthesis pathway from all-trans-beta-carotene to ent-2'-epi-5-deoxystrigol
Arabidopsis thaliana
additional information
the biochemical basis of the shoot branching phenotype is not due to inhibition of enzyme CCD7, but of enzyme CCD8, EC 1.13.11.70
Arabidopsis thaliana
physiological function
biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene
Arabidopsis thaliana
Other publictions for EC 1.13.11.68
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745435
Priya
Molecular modeling and dynami ...
Arabidopsis thaliana
J. Cell. Biochem.
118
2712-2721
2017
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1
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2
2
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745469
Bruno
Enzymatic study on AtCCD4 and ...
Arabidopsis thaliana
J. Exp. Bot.
67
5993-6005
2016
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1
1
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746156
Pan
ZmCCD7/ZpCCD7 encodes a carot ...
Zea mays subsp. parviglumis, Zea mays, Zea mays B73
Planta
243
1407-1418
2016
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2
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2
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6
6
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744876
Harrison
Biochemical characterization ...
Arabidopsis thaliana
FEBS J.
282
3986-4000
2015
-
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1
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-
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1
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1
-
3
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1
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1
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1
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1
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2
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1
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1
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3
3
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744603
Rubio-Moraga
Apical dominance in saffron a ...
Crocus sativus
BMC Plant Biol.
14
171
2014
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1
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744898
Bruno
On the substrate- and stereos ...
Pisum sativum, Arabidopsis thaliana
FEBS Lett.
588
1802-1807
2014
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2
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6
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6
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6
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12
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4
2
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2
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6
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12
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2
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2
2
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746148
Aly
Gene silencing of CCD7 and CC ...
Phelipanche aegyptiaca
Plant Signal. Behav.
9
e29376
2014
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1
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1
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1
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6
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6
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2
2
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721057
Alder
The path from beta-carotene to ...
Arabidopsis thaliana, Oryza sativa, Pisum sativum
Science
335
1348-1351
2012
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3
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4
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720559
Ledger
Modified CAROTENOID CLEAVAGE D ...
Actinidia chinensis
New Phytol.
188
803-813
2010
-
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728478
Vogel
SlCCD7 controls strigolactone ...
Phelipanche ramosa
Plant J.
61
300-311
2010
-
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4
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1
1
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720702
Auldridge
Characterization of three memb ...
Arabidopsis thaliana
Plant J.
45
982-993
2006
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1
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1
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719823
Schwartz
The biochemical characterizati ...
Arabidopsis thaliana
J. Biol. Chem.
279
46940-46945
2004
1
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1
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1
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9
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