Information on EC 1.13.11.68 - 9-cis-beta-carotene 9',10'-cleaving dioxygenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.13.11.68
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RECOMMENDED NAME
GeneOntology No.
9-cis-beta-carotene 9',10'-cleaving dioxygenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
9-cis-beta-carotene + O2 = 9-cis-10'-apo-beta-carotenal + beta-ionone
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
5-deoxystrigol biosynthesis
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Carotenoid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
9-cis-beta-carotene:O2 oxidoreductase (9',10'-cleaving)
Requires Fe2+. The enzyme participates in a pathway leading to biosynthesis of strigolactones, plant hormones involved in promotion of symbiotic associations known as arbuscular mycorrhiza.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
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biosynthesis of strigolactones requires the action of two CCD enzymes, CCD7 and CCD8 (EC 1.13.11.70), which act sequentially on 9-cis-beta-carotene, strigolactone biosynthesis pathway from all-trans-beta-carotene to ent-2'-epi-5-deoxystrigol
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
9'-cis-cryptoxanthin + O2
?
show the reaction diagram
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-
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?
9'-cis-neurosporene + O2
zeta-apo-10'-carotenal + beta-ionone
show the reaction diagram
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low activity
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-
?
9'-cis-prolycopene + O2
zeta-apo-10'-carotenal + beta-ionone
show the reaction diagram
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?
9'-cis-proneurosporene + O2
zeta-apo-10'-carotenal + beta-ionone
show the reaction diagram
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-
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-
?
9-cis-beta-carotene + 02
9-cis-beta-apo-10'-carotenal + beta-ionone
show the reaction diagram
9-cis-beta-carotene + O2
9-cis-10'-apo-beta-carotenal + beta-ionone
show the reaction diagram
9-cis-cryptoxanthin + O2
?
show the reaction diagram
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?
9-cis-lutein + O2
9-cis-3-hydroxy-epsilon-apo-10'-carotenal + 3-hydroxy-beta-ionone
show the reaction diagram
9-cis-zeaxanthin + O2
9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone
show the reaction diagram
9-cis-zeta-carotene + O2
9-cis-zeta-apo-10'-carotenal + beta-ionone
show the reaction diagram
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?
beta-carotene + O2
10'-apo-beta-carotenal + beta-ionone
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
9-cis-beta-carotene + O2
9-cis-10'-apo-beta-carotenal + beta-ionone
show the reaction diagram
9-cis-zeaxanthin + O2
9-cis-3-hydroxy-beta-apo-10'-carotenal + 3-hydroxy-beta-ionone
show the reaction diagram
additional information
?
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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no inhibition by hydroxamic acids
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methanol
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addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0411 - 0.0596
9-cis-beta-carotene
0.1487 - 0.626
9-cis-zeaxanthin
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0.0152
beta-carotene
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pH 7.0, temperature not specified in the publication
additional information
additional information
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
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assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
axillary and apical bud
Manually annotated by BRENDA team
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highest expression level of CCD7
Manually annotated by BRENDA team
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low expression level of CCD8
Manually annotated by BRENDA team
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moderate expression level of CCD8
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Methanol
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addition of methanol to in vitro assays has a stimulatory effect on activity at concentrations up to 25%
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant GST-tagged AtCCD7 from Escherichia coli by glutathione affinity chromatographyy
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed as a thioredoxin and GST fusion proteins in Escherichia coli
gene ccd7, DNA and amino acid sequence determination and anaysis, sequence comparison and phylogenetic tree, transient expression of GFP-tagged ZmCCD7 in maize mesophyll protoplasts indicating localization in the plastid, recombinant expression in Escherichia coli strain BL21-AI
gene ccd7, DNA and amino acid sequence determination and anaysis, sequence comparison and phylogenetic tree, transient expression of GFP-tagged ZpCCD7 in maize mesophyll protoplasts indicating localization in the plastid, recombinant expression in Escherichia coli strain BL21-AI
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gene ccd7, quantitative real-time and RT-PCR enzyme expression analysis
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gene ccd7, recombinant expression in Escherichia coli strain BL21(DE3)
gene ccd7, recombinant expression of GST-tagged AtCCD7 in Escherichia coli
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gene ccd7, sequence comparisons
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information