EC Number |
Reaction |
Reference |
---|
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
active site mapping, catalytic residue Tyr775 in the gyrase half of the Leishmania enzyme is responsible for DNA binding, breakage, and rejoining |
662977 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
active site Y805 |
661093, 662813 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
catalytic residue is Y815, active site structure includes residues Q789, D799, A801, S802, R804, and Y805 |
662598 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
characterization of interactions between topoisomerase II and DNA which occurs after DNA strand passage but before enzyme turnover |
3882 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
enzyme is involved in the chromosome segregation that occurs at the end of the DNA replication |
3869 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
mechanistic models of DNA gyrase |
3813 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
mechanistic studies |
3812 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
metal-dependent general acid-base catalysis, quantum mechanical/molecular mechanical calculations for the topo II religation reaction, substrate-mediated proton transfer pathway, which involves the 3'-OH nucleophile, the reactive phosphate, water, Arg781, and Tyr782, metal A stabilizes the transition states, which is consistent with a two-metal mechanism in topo II, overview |
728001 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
model in which the DNA-bound enzyme acts as an ATP-operated clamp for the capture and transport of a second DNA segment |
3902 |
5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA |
processive mode of the reaction can be shifted to a distributive mode under three different conditions: 1. high ionic strength, above 170 mM, 2. high Mg2+ concentration, above 15 mM, 3. pH-values above 10 in glycine buffer |
3813 |