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Literature summary for 5.6.2.2 extracted from

  • Bromberg, K.D.; Velez-Cruz, R.; Burgin, A.B.; Osheroff, N.
    DNA ligation catalyzed by human topoisomerase II alpha (2004), Biochemistry, 43, 13416-13423.
    View publication on PubMed
Search for more literature for 5.6.2.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae Homo sapiens

Protein Variants

Protein Variants Comment Organism
R804A site-directed mutagenesis, residue next to active site tyrosine, mutation only slightly influences the ligation reaction Homo sapiens
Y805A site-directed mutagenesis, active site residue mutant, mutant shows reduced ligation activity compared to the wild-type enzyme Homo sapiens
Y805C site-directed mutagenesis, active site residue mutant, mutant shows increased ligation activity compared to the wild-type enzyme Homo sapiens
Y805F site-directed mutagenesis, active site residue mutant, mutant shows increased ligation activity compared to the wild-type enzyme Homo sapiens
Y805H site-directed mutagenesis, active site residue mutant, mutant shows increased ligation activity compared to the wild-type enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ preferred divalent cation Homo sapiens
Mg2+ shows 2-2.5fold less activity compared to Ca2+ Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is essential for genomic integrity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP-dependent breakage, passage and rejoining of double-stranded DNA active site Y805 Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 development of an assay method for ligation activity determination independent of DNA cleavage prior to ligation Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dsDNA + ATP + H2O cleavage of dsDNA, religation of dsDNA, ligation by the enzyme of the two strands of a double helix in a nonconcerted fashion, ligation of a nicked oligonucleotide in which the 5'-terminal phosphate at the nick is activated by covalent attachment to 4-nitrophenol, the active site Tyr805 is not important for catalysis of ligation Homo sapiens ?
-
 ?
additional information the enzyme is essential for genomic integrity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
topoisomerase II alpha
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens