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Results 1 - 6 of 6
EC Number Purification (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.165- 696802
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.165gel filtration, anion exchange chromatography, and affinity chromatography on adenosine-agarose 680600
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.165native enzyme 2700fold to homogeneity by ammonium sulfate fractionation, gel filtration, adenosine affinity chromatography, and a second step of gel filtration 682593
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.165partial 696997
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.165the difficulty of solubilization of this membrane-bound labile enzyme is the greatest obstacle to its purification 677650
Display the word mapDisplay the reaction diagram Show all sequences 2.1.1.165the purification is achieved by an 80 to 100% ammonium sulfate precipitation step followed by a high-performance liquid chromatography gel filtration step on a 60 cm by 2.15 cm preparative Bio-Sil SEC-250 column. The column is eluted with 10 mM phosphate buffer, pH 7.0 701215
Results 1 - 6 of 6