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Literature summary for 2.1.1.165 extracted from

  • Wuosmaa, A.M.; Hager, L.P.
    Methyl chloride transferase: a carbocation route for biosynthesis of halometabolites (1990), Science, 249, 160-162.
    View publication on PubMed
Search for more literature for 2.1.1.165

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
 S-adenosyl-L-methionine cosubstrate: bromide Endocladia muricata
5
-
 bromide
-
 Endocladia muricata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000 25000 gel filtration Endocladia muricata

Organism

Organism UniProt Comment Textmining
Endocladia muricata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
the purification is achieved by an 80 to 100% ammonium sulfate precipitation step followed by a high-performance liquid chromatography gel filtration step on a 60 cm by 2.15 cm preparative Bio-Sil SEC-250 column. The column is eluted with 10 mM phosphate buffer, pH 7.0 Endocladia muricata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.000555
-
 partially purified enzyme, synthesis of methyl bromide Endocladia muricata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information fluoride is not a substrate Endocladia muricata ?
-
 ?
S-adenosyl-L-methionine + bromide the rate of production of methyl bromide is 135fold lower than production of methyl iodide Endocladia muricata S-adenosyl-L-homocysteine + methyl bromide
-
 ?
S-adenosyl-L-methionine + chloride the rate of production of methyl chloride is 270fold lower than production of methyl iodide Endocladia muricata S-adenosyl-L-homocysteine + methyl chloride
-
 ?
S-adenosyl-L-methionine + iodide iodide is the preferred substrate Endocladia muricata S-adenosyl-L-homocysteine + methyl iodide
-
 ?

Synonyms

Synonyms Comment Organism
methyl chloride transferase
-
 Endocladia muricata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.6 synthesis of methyl bromide Endocladia muricata

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 40% of maximal activity, pH 9.0: about 35% of maximal activity, at pH greater than 9.2 no methyl bromide synthesis detected Endocladia muricata