BRENDA - Enzyme Database show
show all sequences of 2.1.1.165

Purification and characterization of a monohalomethane-producing enzyme S-adenosyl-L-methionine: halide ion methyltransferase from a marine microalga, Pavlova pinguis

Ohsawa, N.; Tsujita, M.; Morikawa, S.; Itoh, N.; Biosci. Biotechnol. Biochem. 65, 2397-2404 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
1 mM, 16% inhibition, production of methyl iodide
Pavlova pinguis
CN-
100 mM, complete inhibition, production of methyl iodide
Pavlova pinguis
CoCl2
1 mM, 18% inhibition, production of methyl iodide
Pavlova pinguis
dithiothreitol
5 mM, 28% inhibition, production of methyl iodide
Pavlova pinguis
EDTA
5 mM, 32% inhibition, production of methyl iodide
Pavlova pinguis
HS-
10 mM, 84% inhibition, production of methyl iodide
Pavlova pinguis
MgSO4
1 mM, 25% inhibition, production of methyl iodide
Pavlova pinguis
monoiodoacetate
5 mM, 28% inhibition, production of methyl iodide
Pavlova pinguis
additional information
activity is not inhibited by high iodide concentrations
Pavlova pinguis
NaN3
5 mM, 32% inhibition, production of methyl iodide
Pavlova pinguis
NiCl2
1 mM, 38% inhibition, production of methyl iodide
Pavlova pinguis
PCMB
0.5 mM, 30% inhibition, production of methyl iodide
Pavlova pinguis
SCN-
10 mM, 60% inhibition, production of methyl iodide
Pavlova pinguis
ZnCl2
1 mM, 14% inhibition, production of methyl iodide
Pavlova pinguis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
S-adenosyl-L-methionine
30°C
Pavlova pinguis
12
-
Iodide
30°C
Pavlova pinguis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
various metal ions have no significant effect on methyl iodide production, suggesting that the enzyme does not require metal ions
Pavlova pinguis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
gel filtration
Pavlova pinguis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pavlova pinguis
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pavlova pinguis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0018
-
-
Pavlova pinguis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme also catalyzes the methylation of HS- to methyl mercaptan (EC 2.1.1.9)
696802
Pavlova pinguis
?
-
-
-
-
S-adenosyl-L-methionine + bromide
-
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + methyl bromide
-
-
-
?
S-adenosyl-L-methionine + chloride
-
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + methyl chloride
-
-
-
?
S-adenosyl-L-methionine + iodide
the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + methyl iodide
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Pavlova pinguis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pavlova pinguis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.4
10
pH 5.4: about 50% of maximal activity, pH 10.0: about 80% of maximal activity
Pavlova pinguis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pavlova pinguis
-
-
5.3
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
1 mM, 16% inhibition, production of methyl iodide
Pavlova pinguis
CN-
100 mM, complete inhibition, production of methyl iodide
Pavlova pinguis
CoCl2
1 mM, 18% inhibition, production of methyl iodide
Pavlova pinguis
dithiothreitol
5 mM, 28% inhibition, production of methyl iodide
Pavlova pinguis
EDTA
5 mM, 32% inhibition, production of methyl iodide
Pavlova pinguis
HS-
10 mM, 84% inhibition, production of methyl iodide
Pavlova pinguis
MgSO4
1 mM, 25% inhibition, production of methyl iodide
Pavlova pinguis
monoiodoacetate
5 mM, 28% inhibition, production of methyl iodide
Pavlova pinguis
additional information
activity is not inhibited by high iodide concentrations
Pavlova pinguis
NaN3
5 mM, 32% inhibition, production of methyl iodide
Pavlova pinguis
NiCl2
1 mM, 38% inhibition, production of methyl iodide
Pavlova pinguis
PCMB
0.5 mM, 30% inhibition, production of methyl iodide
Pavlova pinguis
SCN-
10 mM, 60% inhibition, production of methyl iodide
Pavlova pinguis
ZnCl2
1 mM, 14% inhibition, production of methyl iodide
Pavlova pinguis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.012
-
S-adenosyl-L-methionine
30°C
Pavlova pinguis
12
-
Iodide
30°C
Pavlova pinguis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
various metal ions have no significant effect on methyl iodide production, suggesting that the enzyme does not require metal ions
Pavlova pinguis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
gel filtration
Pavlova pinguis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pavlova pinguis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.0018
-
-
Pavlova pinguis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
the enzyme also catalyzes the methylation of HS- to methyl mercaptan (EC 2.1.1.9)
696802
Pavlova pinguis
?
-
-
-
-
S-adenosyl-L-methionine + bromide
-
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + methyl bromide
-
-
-
?
S-adenosyl-L-methionine + chloride
-
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + methyl chloride
-
-
-
?
S-adenosyl-L-methionine + iodide
the enzyme is strictly dependent on S-adenosyl-L-methionine as a methyl donor
696802
Pavlova pinguis
S-adenosyl-L-homocysteine + methyl iodide
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 29000, SDS-PAGE
Pavlova pinguis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Pavlova pinguis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.4
10
pH 5.4: about 50% of maximal activity, pH 10.0: about 80% of maximal activity
Pavlova pinguis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pavlova pinguis
-
-
5.3
Other publictions for EC 2.1.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
696997
Itoh
Involvement of S-adenosylmethi ...
Raphanus sativus
BMC Plant Biol.
9
116
2009
-
-
1
-
-
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4
-
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2
1
-
1
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1
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1
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5
1
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1
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1
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1
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1
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5
1
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-
-
-
-
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-
698496
Bayer
Synthesis of methyl halides fr ...
Batis maritima, Brassica rapa, Burkholderia pseudomallei, Burkholderia thailandensis, Oryza sativa, Paraburkholderia phytofirmans, Paraburkholderia xenovorans, Vitis vinifera
J. Am. Chem. Soc.
131
6508-6515
2009
-
8
8
-
-
-
-
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16
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21
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8
8
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21
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698916
Nagatoshi Y
Arabidopsis HARMLESS TO OZONE ...
Arabidopsis thaliana
J. Biol. Chem.
284
19301-19309
2009
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3
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700702
Nagatoshi. Y.; Nakamura
-
Characterization of three hali ...
Arabidopsis thaliana
Plant Biotechnol.
24
503-506
2007
-
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1
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-
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2
3
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3
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3
3
9
1
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3
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3
3
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3
3
9
3
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679357
Rhew
Genetic control of methyl hali ...
Arabidopsis thaliana
Curr. Biol.
13
1809-1813
2003
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1
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695730
Amachi
Bacteria mediate methylation o ...
Rhizobium sp., Rhizobium sp. MRCD 19
Appl. Environ. Microbiol.
67
2718-2722
2001
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-
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1
2
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2
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2
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1
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1
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696802
Ohsawa
Purification and characterizat ...
Pavlova pinguis
Biosci. Biotechnol. Biochem.
65
2397-2404
2001
-
-
-
-
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14
2
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1
1
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2
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1
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1
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4
1
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1
1
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1
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14
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2
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1
1
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1
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1
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4
1
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1
1
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1
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682595
Ni
Expression of Batis maritima m ...
Batis maritima
Proc. Natl. Acad. Sci. USA
96
3611-3615
1999
1
-
1
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1
8
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1
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1
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5
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4
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3
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8
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4
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3
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677650
Saxena
Biochemical characterization o ...
Phellinus pomaceus
Appl. Environ. Microbiol.
64
2831-2835
1998
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1
4
1
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3
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4
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1
1
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682593
Ni
cDNA cloning of Batis maritima ...
Batis maritima
Proc. Natl. Acad. Sci. USA
95
12866-12871
1998
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1
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1
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3
1
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3
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1
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1
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4
1
1
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1
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1
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700660
Itoh
-
Formation and emission of mono ...
Papenfussiella kuromo, Pavlova gyrans, Sargassum horneri
Phytochemistry
45
67-73
1997
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2
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3
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8
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1
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1
1
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680600
Attieh
Purification and characterizat ...
Brassica oleracea
J. Biol. Chem.
270
9250-9257
1995
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2
4
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2
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2
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1
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700704
Saini
-
Biosynthesis of halomethanes a ...
Brassica oleracea
Plant Cell Environ.
18
1027-1033
1995
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701215
Wuosmaa
Methyl chloride transferase: a ...
Endocladia muricata
Science
249
160-162
1990
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2
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