Inhibitors | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-homocysteine | 50% inhibition at equimolar concentrations of S-adenosyl-L-homocysteine and S-adenosyl-L-methionine | Phellinus pomaceus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0045 | - |
S-adenosyl-L-methionine | pH 6.8, 22°C | Phellinus pomaceus | |
0.25 | - |
Iodide | pH 6.8, 22°C | Phellinus pomaceus | |
10 | - |
bromide | pH 6.8, 22°C | Phellinus pomaceus | |
300 | - |
chloride | pH 6.8, 22°C | Phellinus pomaceus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound to | Phellinus pomaceus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + chloride | Phellinus pomaceus | the enzyme is responsible for the massive amounts of CH3Cl produced by this fungus | S-adenosyl-L-homocysteine + methyl chloride | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phellinus pomaceus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the difficulty of solubilization of this membrane-bound labile enzyme is the greatest obstacle to its purification | Phellinus pomaceus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | the fungus is cultured in undisturbed glucose mycological peptone liquid medium | Phellinus pomaceus | - |
Storage Stability | Organism |
---|---|
-20°C to 4°C, partially purified enzyme, complete loss of activity overnight, also in the presence of protease inhibitors | Phellinus pomaceus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + bromide | Vmax/Km for bromide is 17fold lower than Vmax/Km for iodide | Phellinus pomaceus | S-adenosyl-L-homocysteine + methyl bromide | - |
? | |
S-adenosyl-L-methionine + chloride | the enzyme is responsible for the massive amounts of CH3Cl produced by this fungus | Phellinus pomaceus | S-adenosyl-L-homocysteine + methyl chloride | - |
? | |
S-adenosyl-L-methionine + chloride | Vmax/Km for chloride is 709fold lower than Vmax/Km for iodide | Phellinus pomaceus | S-adenosyl-L-homocysteine + methyl chloride | - |
? | |
S-adenosyl-L-methionine + iodide | iodide is the preferred substrate | Phellinus pomaceus | S-adenosyl-L-homocysteine + methyl iodide | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.2 | - |
Phellinus pomaceus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 35% of maximal activity, pH 8.0: about 75% of maximal activity | Phellinus pomaceus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | specific for, natural methyl donor | Phellinus pomaceus |