EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
---|
3.4.24.74 | 19000 |
- |
1 * 19000, SDS-PAGE |
31497 |
3.4.24.74 | 20600 |
- |
1 * 20600, SDS-PAGE |
31496 |
3.4.24.74 | -999 |
- |
analysis of BFT-2 by native PAGE reveals bands at 36000 Da, 90000 Da and 200000 Da. Both the 36000 Da and higher-molecular-mass bands are biologically active when tested in the HT29/C1 cell assay |
651483 |
3.4.24.74 | 20600 |
- |
cleaved to its enzymatically active secreted form |
668836 |
3.4.24.74 | 20000 |
- |
gel electrophoresis and Western blot analysis, mature processed BFT in all mutants |
668924 |
3.4.24.74 | 44000 |
- |
gel electrophoresis and Western blot analysis, unprocessed BFT in mutants H348D and M366R |
668924 |
3.4.24.74 | 18000 |
22000 |
gel fitration |
31497 |
3.4.24.74 | 44400 |
- |
preproprotein |
668836 |
3.4.24.74 | 44000 |
- |
unprocessed enzyme, Western blot analysis of whole cell lysates in preperations of strain NCTC 9343 expressing wild type BFT |
668932 |
3.4.24.74 | 20000 |
- |
Western blot analysis of cell-free culture supernatants of strain NCTC 9343 expressing mutants BFT-C2, BFT-C4 and BFT-A394E, and of whole cell lysates in preperations of strain NCTC 9343 expressing wild type BFT |
668932 |