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Literature summary for 3.4.24.74 extracted from

  • Franco, A.A.; Buckwold, S.L.; Shin, J.W.; Ascon, M.; Sears, C.L.
    Mutation of the zinc-binding metalloprotease motif affects Bacteroides fragilis toxin activity but does not affect propeptide processing (2005), Infect. Immun., 73, 5273-5277.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
additional information single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro Bacteroides fragilis

Cloned(Commentary)

Cloned (Comment) Organism
expression of H348D in Escherichia coli Bacteroides fragilis

Protein Variants

Protein Variants Comment Organism
E349A expression of BFT similar to that of wild type Bacteroides fragilis
G355R expression of BFT similar to that of wild type Bacteroides fragilis
H348D smaller amounts of BFT compared to wild type Bacteroides fragilis
H352Y expression of BFT similar to that of wild type Bacteroides fragilis
H358Y expression of BFT similar to that of wild type Bacteroides fragilis
M366R smaller amounts of BFT compared to wild type Bacteroides fragilis

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Bacteroides fragilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
gel electrophoresis and Western blot analysis, mature processed BFT in all mutants Bacteroides fragilis
44000
-
gel electrophoresis and Western blot analysis, unprocessed BFT in mutants H348D and M366R Bacteroides fragilis

Organism

Organism UniProt Comment Textmining
Bacteroides fragilis
-
strain 086-5443-2-2, strain NCTC 9343
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
E-cadherin + H2O
-
Bacteroides fragilis ?
-
?

Synonyms

Synonyms Comment Organism
Bacteroides fragilis toxin
-
Bacteroides fragilis
BFT
-
Bacteroides fragilis