Application | Comment | Organism |
---|---|---|
additional information | single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro | Bacteroides fragilis |
Cloned (Comment) | Organism |
---|---|
expression of H348D in Escherichia coli | Bacteroides fragilis |
Protein Variants | Comment | Organism |
---|---|---|
E349A | expression of BFT similar to that of wild type | Bacteroides fragilis |
G355R | expression of BFT similar to that of wild type | Bacteroides fragilis |
H348D | smaller amounts of BFT compared to wild type | Bacteroides fragilis |
H352Y | expression of BFT similar to that of wild type | Bacteroides fragilis |
H358Y | expression of BFT similar to that of wild type | Bacteroides fragilis |
M366R | smaller amounts of BFT compared to wild type | Bacteroides fragilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Bacteroides fragilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
gel electrophoresis and Western blot analysis, mature processed BFT in all mutants | Bacteroides fragilis |
44000 | - |
gel electrophoresis and Western blot analysis, unprocessed BFT in mutants H348D and M366R | Bacteroides fragilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides fragilis | - |
strain 086-5443-2-2, strain NCTC 9343 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
E-cadherin + H2O | - |
Bacteroides fragilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bacteroides fragilis toxin | - |
Bacteroides fragilis |
BFT | - |
Bacteroides fragilis |