EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate  |
Reference |
|---|
   3.1.4.53 | 0.00025 |
- |
3',5'-cAMP |
30°C, pH 8.0, PDE7 |
649785 |
| 3.1.4.53 | 0.0007 |
- |
3',5'-cAMP |
mutant enzyme T748A, in 20 mM Tris-HCl, pH 7.5, 4 mM MnCl2, at 24°C |
690916 |
| 3.1.4.53 | 0.00099 |
- |
3',5'-cAMP |
- |
668091 |
| 3.1.4.53 | 0.0018 |
- |
3',5'-cAMP |
wild type PDE8A1 catalytic domain, in 20 mM Tris-HCl, pH 7.5, 4 mM MnCl2, at 24°C |
690916 |
| 3.1.4.53 | 0.0025 |
- |
3',5'-cAMP |
30°C, pH 8.0, PDE4 |
649785 |
| 3.1.4.53 | 0.0032 |
- |
3',5'-cAMP |
- |
135278 |
| 3.1.4.53 | 0.0033 |
- |
3',5'-cAMP |
PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C |
692369 |
| 3.1.4.53 | 0.005 |
- |
3',5'-cAMP |
PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C |
692369 |
| 3.1.4.53 | 0.0067 |
- |
3',5'-cAMP |
the addition of FeCl2 does not significantly influence substrate affinity of CdpA increases the rate of the 5'-AMP production, pH and temperature not specified in the publication |
712298 |
| 3.1.4.53 | 0.00698 |
- |
3',5'-cAMP |
- |
668091 |
