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Literature summary for 3.1.4.53 extracted from

  • Kimura, Y.; Okazaki, N.; Takegawa, K.
    Enzymatic characteristics of two novel Myxococcus xanthus enzymes, PdeA and PdeB, displaying 3',5'- and 2'3'-cAMP phosphodiesterase, and phosphatase activities (2009), FEBS Lett., 583, 443-448.
    View publication on PubMed
Search for more literature for 3.1.4.53

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Myxococcus xanthus

Inhibitors

Inhibitors Comment Organism Structure
EDTA PdeA and PdeB show 39% residual activity, respectively, for 3',5'-cAMP hydrolysis at 0.1 mM EDTA Myxococcus xanthus
additional information he 3',5'-phosphodiesterase activities of PdeA and PdeB are not inhibited by theophylline, 3-isobuthyl-1-methylxanthine, and beta-glycerophosphate Myxococcus xanthus
O-phospho-L-serine PdeA and PdeB show 59% and 73% residual activity, respectively, for 3',5'-cAMP hydrolysis at 5 mM phosphoserine Myxococcus xanthus
O-phospho-L-tyrosine PdeA and PdeB show 44% and 52% residual activity, respectively, for 3',5'-cAMP hydrolysis at 5 mM phosphotyrosine Myxococcus xanthus
orthovanadate PdeA and PdeB show 26% and 29% residual activity, respectively, for 3',5'-cAMP hydrolysis at 1 mM orthovanadate Myxococcus xanthus
Zn2+ the 3',5'-phosphodiesterase enzyme activities of PdeA and PdeB are reduced to 24% and 28%, respectively, by 0.05 mM Zn2+ at pH 8.0 in 50 mM Tris-HCl buffer Myxococcus xanthus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0033
-
 3',5'-cAMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.005
-
 3',5'-cAMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ the 3',5'-phosphodiesterase enzyme activities of PdeA and PdeB are stimulated 3.2fold and 1.98old, respectively, by 0.05 mM Co2+ at pH 8.0 in 50 mM Tris-HCl buffer Myxococcus xanthus
Mn2+ the 3',5'-phosphodiesterase enzyme activities of PdeA and PdeB are stimulated 4fold and 2.14old, respectively, by 0.05 mM Mn2+ at pH 8.0 in 50 mM Tris-HCl buffer Myxococcus xanthus
additional information the 3',5'-phosphodiesterase enzyme activities of PdeA and PdeB are not stimulated by 0.05 mM Ca2+, Mg2+, Fe2+, and Fe3+ Myxococcus xanthus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 PdeA, SDS-PAGE Myxococcus xanthus
29000
-
 PdeA, calculated from amino acid sequence Myxococcus xanthus
31000
-
 PdeB, calculated from amino acid sequence Myxococcus xanthus
34000
-
 PdeB, SDS-PAGE Myxococcus xanthus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3',5'-cAMP + H2O Myxococcus xanthus
-
 5'-AMP the reaction product, 5'-AMP, is further dephosphorylated to adenosine by PdeA and PdeB ?
additional information Myxococcus xanthus Myxococcus xanthus PdeA and PdeB, enzymes hydrolyze 3',5'- and 2',3'-cyclic AMP to adenosine, and also demonstrate phosphatase activity toward nucleoside 5'-tri-, 5'-di-, 5'- and 3'-monophosphates with highest activities for nucleoside 5'-monophosphates. PdeA and PdeB also show high phosphomonoesterase activities against 50-UMP, 3'-AMP, and 3'-GMP, low activities against 5'-dAMP, and no activities toward 2'-AMP and 2'-GMP ?
-
 ?

Organism

Organism UniProt Comment Textmining
Myxococcus xanthus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon CellThru column chromatography, gel filtration Myxococcus xanthus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3',5'-cAMP + H2O
-
 Myxococcus xanthus 5'-AMP the reaction product, 5'-AMP, is further dephosphorylated to adenosine by PdeA and PdeB ?
additional information Myxococcus xanthus PdeA and PdeB, enzymes hydrolyze 3',5'- and 2',3'-cyclic AMP to adenosine, and also demonstrate phosphatase activity toward nucleoside 5'-tri-, 5'-di-, 5'- and 3'-monophosphates with highest activities for nucleoside 5'-monophosphates. PdeA and PdeB also show high phosphomonoesterase activities against 50-UMP, 3'-AMP, and 3'-GMP, low activities against 5'-dAMP, and no activities toward 2'-AMP and 2'-GMP Myxococcus xanthus ?
-
 ?

Synonyms

Synonyms Comment Organism
PdeA
-
 Myxococcus xanthus
PdeB
-
 Myxococcus xanthus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 the optimum temperatures for the 3',5'-cAMP phosphodiesterase activity of PdeA is 40°C Myxococcus xanthus
50
-
 the optimum temperatures for the 3',5'-cAMP phosphodiesterase activity of PdeB is 50°C Myxococcus xanthus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 55 heat treatment at 50°C or 55°C for 5 min slightly activates the phosphodiesterase activity of PdeB against 3',5'-cAMP by about 1.2fold of the control while PdeA activity is not activated by the heat treatment Myxococcus xanthus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00093
-
 3',5'-cAMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.00096
-
 3',5'-cAMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5 PdeA and PdeB have slightly alkaline pH optima (pH 7.5-8.5) for 3',5'-cAMP hydrolysis in 0.1 M Tris-HCl buffer Myxococcus xanthus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 PdeB shows no 3',5'-cAMP phosphodiesterase activity at or below pH 6.0, PdeA and PdeB activities are strongly inhibited by 0.1 M sodium phosphate buffer (pH 6.0 and 7.0) Myxococcus xanthus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
 3',5'-cAMP PdeB, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus
0.29
-
 3',5'-cAMP PdeA, in 50 mM Tris-HCl, pH 8.0, 0.05 mM MnCl2, at 40°C Myxococcus xanthus