Information on EC 3.1.3.103 - 3-deoxy-D-glycero-D-galacto-nononate 9-phosphatase

for references in articles please use BRENDA:EC3.1.3.103
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The expected taxonomic range for this enzyme is: Bacteroides thetaiotaomicron

EC NUMBER
COMMENTARY hide
3.1.3.103
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RECOMMENDED NAME
GeneOntology No.
3-deoxy-D-glycero-D-galacto-nononate 9-phosphatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O = 3-deoxy-D-glycero-D-galacto-nononate + phosphate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
3-deoxy-D-glycero-D-galacto-nononate 9-phosphohydrolase
The enzyme, characterized from the bacterium Bacteroides thetaiotaomicron, is part of the biosynthesis pathway of the sialic acid 3-deoxy-D-glycero-D-galacto-non-2-ulopyranosonate (Kdn). Kdn is abundant in extracellular glycoconjugates of lower vertebrates such as fish and amphibians, but is also found in the capsular polysaccharides of bacteria that belong to the Bacteroides genus.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-dehydro-3-deoxy-D-manno-octulosonic acid 8-phosphate + H2O
2-dehydro-3-deoxy-D-manno-octulosonic acid + phosphate
show the reaction diagram
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
3-deoxy-D-glycero-D-galacto-nononate + phosphate
show the reaction diagram
N-acetylneuraminic acid 9-phosphate + H2O
N-acetylneuraminic acid + phosphate
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
3-deoxy-D-glycero-D-galacto-nononate + phosphate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.31
2-dehydro-3-deoxy-D-manno-octulosonic acid 8-phosphate
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pH 7, 25°C
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0.1 - 0.41
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
0.12
N-acetylneuraminic acid 9-phosphate
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pH 7, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
2-dehydro-3-deoxy-D-manno-octulosonic acid 8-phosphate
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pH 7, 25°C
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0.026 - 1.2
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
0.73
N-acetylneuraminic acid 9-phosphate
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pH 7, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06 - 12
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structures of BT_1713 liganded to the cofactor Mg2+ and complexed with tungstate or VO3-/Neu5Ac, to 1.1, 1.85, and 1.63 A resolution, respectively. The active site lies at the subunit interface and Arg64, Lys67, and Glu56 are the key residues involved in sugar binding essential for catalytic function
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E56A
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200fold decrease in catalytic efficiency
R64A
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loss of catalytic activity
S37A
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2fold decrease in catalytic efficiency
T34A
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20fold decrease in catalytic efficiency
E56A
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200fold decrease in catalytic efficiency
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R64A
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loss of catalytic activity
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S37A
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2fold decrease in catalytic efficiency
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T34A
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20fold decrease in catalytic efficiency
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