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Literature summary for 3.1.3.103 extracted from

  • Lu, Z.; Wang, L.; Dunaway-Mariano, D.; Allen, K.N.
    Structure-function analysis of 2-keto-3-deoxy-D-glycero-D-galactonononate-9-phosphate phosphatase defines specificity elements in type C0 haloalkanoate dehalogenase family members (2009), J. Biol. Chem., 284, 1224-1233.
    View publication on PubMedView publication on EuropePMC
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Crystallization (Commentary)

Crystallization (Comment) Organism
structures of BT_1713 liganded to the cofactor Mg2+ and complexed with tungstate or VO3-/Neu5Ac, to 1.1, 1.85, and 1.63 A resolution, respectively. The active site lies at the subunit interface and Arg64, Lys67, and Glu56 are the key residues involved in sugar binding essential for catalytic function Bacteroides thetaiotaomicron

Protein Variants

Protein Variants Comment Organism
E56A 200fold decrease in catalytic efficiency Bacteroides thetaiotaomicron
R64A loss of catalytic activity Bacteroides thetaiotaomicron
S37A 2fold decrease in catalytic efficiency Bacteroides thetaiotaomicron
T34A 20fold decrease in catalytic efficiency Bacteroides thetaiotaomicron

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate wild-type, pH 7.0, 37°C Bacteroides thetaiotaomicron
0.18
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant T34A, pH 7.0, 37°C Bacteroides thetaiotaomicron
0.2
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant S37A, pH 7.0, 37°C Bacteroides thetaiotaomicron
0.41
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant E56A, pH 7.0, 37°C Bacteroides thetaiotaomicron

Organism

Organism UniProt Comment Textmining
Bacteroides thetaiotaomicron Q8A712
-
-
Bacteroides thetaiotaomicron DSM 2079 Q8A712
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron 3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
 ?
3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + H2O
-
 Bacteroides thetaiotaomicron DSM 2079 3-deoxy-D-glycero-D-galacto-nononate + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
BT_1713
-
 Bacteroides thetaiotaomicron

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.026
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant E56A, pH 7.0, 37°C Bacteroides thetaiotaomicron
0.09
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant T34A, pH 7.0, 37°C Bacteroides thetaiotaomicron
1.2
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate wild-type, pH 7.0, 37°C Bacteroides thetaiotaomicron
1.2
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant S37A, pH 7.0, 37°C Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.06
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant E56A, pH 7.0, 37°C Bacteroides thetaiotaomicron
0.5
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant T34A, pH 7.0, 37°C Bacteroides thetaiotaomicron
6
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate mutant S37A, pH 7.0, 37°C Bacteroides thetaiotaomicron
12
-
 3-deoxy-D-glycero-D-galacto-nononate 9-phosphate wild-type, pH 7.0, 37°C Bacteroides thetaiotaomicron