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EC Tree
IUBMB Comments The enzyme participates in the thiamine biosynthesis pathway in eukaryotes and a few prokaryotes. These organisms lack EC 2.7.4.16, thiamine-phosphate kinase, and need to convert thiamine phosphate to thiamine diphosphate, the active form of the vitamin, by first removing the phosphate group, followed by diphosphorylation by EC 2.7.6.2, thiamine diphosphokinase.
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
thiamine monophosphatase, thiamin-binding protein, thiamine-repressible acid phosphatase, thmpase, thiamine phosphatase, thiamine phosphate phosphatase, tdp/tmp phosphatase,
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hydroxyethylthiazole kinase
thiamin-repressible acid phosphatase
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thiamine phosphate phosphatase
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thiamine-(di)phosphate phosphatase
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thiamine-repressible acid phosphatase
hydroxyethylthiazole kinase
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hydroxyethylthiazole kinase
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thiamin-binding protein
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thiamin-binding protein
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thiamine monophosphatase
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thiamine monophosphatase
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thiamine-repressible acid phosphatase
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thiamine-repressible acid phosphatase
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thiamine-repressible acid phosphatase
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TMPase
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thiamine phosphate + H2O = thiamine + phosphate
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thiamine phosphate phosphohydrolase
The enzyme participates in the thiamine biosynthesis pathway in eukaryotes and a few prokaryotes. These organisms lack EC 2.7.4.16, thiamine-phosphate kinase, and need to convert thiamine phosphate to thiamine diphosphate, the active form of the vitamin, by first removing the phosphate group, followed by diphosphorylation by EC 2.7.6.2, thiamine diphosphokinase.
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4-nitrophenyl phosphate + H2O
4-nitrophenol + phosphate
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100% activity
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?
FMN + H2O
?
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73% activity compared to 4-nitrophenyl phosphate
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?
L-phosphotyrosine + H2O
phosphate + L-tyrosine
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32% activity compared to 4-nitrophenyl phosphate
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?
thiamine + phosphate
thiamine phosphate + H2O
thiamine diphosphate + H2O
thiamine phosphate + phosphate
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preferred substrate
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?
thiamine phosphate + H2O
thiamine + phosphate
additional information
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less than 1% activity with pyridoxal 5'-phosphate, beta-glycerophosphate, D-glucose 6-phosphate, D-fructose 1,6-diphosphate, inosine 5'-monophosphate, or adenosine 5'-monophosphate
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?
thiamine + phosphate
thiamine phosphate + H2O
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?
thiamine + phosphate
thiamine phosphate + H2O
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?
thiamine phosphate + H2O
thiamine + phosphate
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-
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?
thiamine phosphate + H2O
thiamine + phosphate
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18% activity compared to 4-nitrophenyl phosphate
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-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
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?
thiamine phosphate + H2O
thiamine + phosphate
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-
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?
thiamine phosphate + H2O
thiamine + phosphate
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-
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?
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thiamine + phosphate
thiamine phosphate + H2O
thiamine diphosphate + H2O
thiamine phosphate + phosphate
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preferred substrate
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-
?
thiamine phosphate + H2O
thiamine + phosphate
thiamine + phosphate
thiamine phosphate + H2O
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-
-
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?
thiamine + phosphate
thiamine phosphate + H2O
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-
-
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?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
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-
?
thiamine phosphate + H2O
thiamine + phosphate
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-
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?
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Mg2+
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membrane-bound enzyme activity is enhanced 1.7fold by 5 mM Mg2+, while the soluble enzyme form is independent from Mg2+
additional information
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Mg2+, Mn2+ or Ca2+ do not affect the enzyme activity
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levamisole
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membrane-bound enzyme is strongly inhibited by levamisole in uncompetitive manner while the soluble enzyme form is insensitive to levamisole
thiamine
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the enzyme is repressed completely by excess thiamine (0.01 mM)
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Hepatic Encephalopathy
Thiamine phosphatases in human brain: regional alterations in patients with alcoholic cirrhosis.
Neuralgia
Prostatic acid phosphatase (PAP) is the main acid phosphatase with 5'-ectonucleotidase activity in the male mouse saliva and regulates salivation.
Peripheral Nerve Injuries
Spared nerve injury model of neuropathic pain in the mouse: a behavioral and anatomic analysis.
Thiamine Deficiency
Enzyme-cytochemical study of small ganglion cells in experimental thiamine deficiency: concerning the pain mechanism.
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0.036
thiamine diphosphate
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at pH 5.0, temperature not specified in the publication
0.049 - 0.7
thiamine phosphate
0.049
thiamine phosphate
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at pH 5.0, temperature not specified in the publication
0.6
thiamine phosphate
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membrane-bound enzyme form, at pH 9.0, temperature not specified in the publication
0.7
thiamine phosphate
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soluble enzyme form, at pH 6.0, temperature not specified in the publication
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brenda
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brenda
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brenda
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UniProt
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brenda
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brenda
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TENAC_ARATH
617
0
68964
Swiss-Prot
Mitochondrion (Reliability: 3 )
PPSP3_ARATH
245
0
28053
Swiss-Prot
other Location (Reliability: 3 )
PPA2_SCHPO
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
463
0
52119
Swiss-Prot
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24000
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2 * 24000, SDS-PAGE
56000
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x * 56000, SDS-PAGE
17800
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gel filtration
17800
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x * 17800, soluble enzyme form, SDS-PAGE
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homodimer
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2 * 24000, SDS-PAGE
?
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x * 17800, soluble enzyme form, SDS-PAGE
?
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x * 56000, SDS-PAGE
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glycoprotein
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55
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the enzyme is inactivated after 10 min at 55°C
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ammonium sulfate precipitation, Ni-NTA column chromatography, and Servacel P-23 chromatography, and Sephadex G-50 gel filtration
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DEAE-cellulose column chromatography
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Toyopearl HW-55 column chromatography and gel filtration
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expressed in Escherichia coli JA 221 cells
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the enzyme activity is decreased when the seedlings are grown under the illumination
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the enzyme activity is significantly enhanced in Alzheimer's disease patients than those in control subjects
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the enzyme is highly expressed during thiamine starvation
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Nosaka, K.; Nishimura, H.; Iwashima, A.
Identity of soluble thiamin-binding protein with thiamin-repressible acid phosphatase in Saccharomyces cerevisiae
Biochim. Biophys. Acta
967
49-55
1988
Saccharomyces cerevisiae, Saccharomyces cerevisiae X2180-1A
brenda
Yang, J.; Schweingruber, M.
The structural gene coding for thiamin-repressible acid phosphatase in Schizosaccharomyces pombe
Curr. Genet.
18
269-272
1990
Schizosaccharomyces pombe (Q01682)
brenda
Schweingruber, A.; Dlugonski, J.; Edenharter, E.; Schweingruber, M.
Thiamine in Schizosaccharomyces pombe: dephosphorylation, intracellular pool, biosynthesis and transport
Curr. Genet.
19
249-254
1991
Schizosaccharomyces pombe (Q01682)
brenda
Sanemori, H.; Egi, Y.; Kawasaki, T.
Pathway of thiamine pyrophosphate synthesis in Micrococcus denitrificans
J. Bacteriol.
126
1030-1036
1976
Escherichia coli, Paracoccus denitrificans
brenda
Rapala-Kozik, M.; Golda, A.; Kujda, M.
Enzymes that control the thiamine diphosphate pool in plant tissues. Properties of thiamine pyrophosphokinase and thiamine-(di)phosphate phosphatase purified from Zea mays seedlings
Plant Physiol. Biochem.
47
237-242
2009
Zea mays
brenda
Kolos, I.; Makarchikov, A.
Identification of thiamine monophosphate hydrolyzing enzymes in chicken liver
Ukr. Biochem. J.
86
39-49
2014
Gallus gallus
brenda
Nahas, J.V.; Iosue, C.L.; Shaik, N.F.; Selhorst, K.; He, B.Z.; Wykoff, D.D.
Dynamic changes in yeast phosphatase families allow for specialization in phosphate and thiamine starvation
G3 (Bethesda)
8
2333-2343
2018
[Candida] glabrata
brenda
Pan, X.; Sang, S.; Fei, G.; Jin, L.; Liu, H.; Wang, Z.; Wang, H.; Zhong, C.
Enhanced activities of blood thiamine diphosphatase and monophosphatase in Alzheimer's disease
PLoS ONE
12
e0167273
2017
Homo sapiens
brenda
Kolas, I.; Makarchikov, A.
Copurification of chicken liver soluble thiamine monophosphatase and low molecular weight acid phosphatase
Ukr. Biochem. J.
89
13-21
2017
Gallus gallus
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