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Literature summary for 3.1.3.100 extracted from

  • Rapala-Kozik, M.; Golda, A.; Kujda, M.
    Enzymes that control the thiamine diphosphate pool in plant tissues. Properties of thiamine pyrophosphokinase and thiamine-(di)phosphate phosphatase purified from Zea mays seedlings (2009), Plant Physiol. Biochem., 47, 237-242.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
thiamine diphosphate at pH 5.0, temperature not specified in the publication Zea mays
0.049
-
thiamine phosphate at pH 5.0, temperature not specified in the publication Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
2 * 24000, SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
thiamine diphosphate + H2O Zea mays preferred substrate thiamine phosphate + phosphate
-
?
thiamine phosphate + H2O Zea mays
-
thiamine + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiamine diphosphate + H2O preferred substrate Zea mays thiamine phosphate + phosphate
-
?
thiamine phosphate + H2O
-
Zea mays thiamine + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 24000, SDS-PAGE Zea mays

Synonyms

Synonyms Comment Organism
TDP/TMP phosphatase
-
Zea mays
thiamine-(di)phosphate phosphatase
-
Zea mays

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Zea mays

Expression

Organism Comment Expression
Zea mays the enzyme activity is decreased when the seedlings are grown under the illumination down