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Literature summary for 3.1.3.100 extracted from
- Identification of thiamine monophosphate hydrolyzing enzymes in chicken liver (2014), Ukr. Biochem. J., 86, 39-49.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| levamisole | membrane-bound enzyme is strongly inhibited by levamisole in uncompetitive manner while the soluble enzyme form is insensitive to levamisole | Gallus gallus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
thiamine phosphate | membrane-bound enzyme form, at pH 9.0, temperature not specified in the publication | Gallus gallus | |
| 0.7 | - |
thiamine phosphate | soluble enzyme form, at pH 6.0, temperature not specified in the publication | Gallus gallus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Gallus gallus | 16020 | - |
| soluble | - |
Gallus gallus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | membrane-bound enzyme activity is enhanced 1.7fold by 5 mM Mg2+, while the soluble enzyme form is independent from Mg2+ | Gallus gallus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17800 | - |
x * 17800, soluble enzyme form, SDS-PAGE | Gallus gallus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine phosphate + H2O | Gallus gallus | - |
thiamine + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gallus gallus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Toyopearl HW-55 column chromatography and gel filtration | Gallus gallus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Gallus gallus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thiamine phosphate + H2O | - |
Gallus gallus | thiamine + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 17800, soluble enzyme form, SDS-PAGE | Gallus gallus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TMPase | - |
Gallus gallus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
soluble enzyme form | Gallus gallus |
| 9 | - |
membrane enzyme form | Gallus gallus |
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