Information on EC 1.8.98.5 - H2:CoB-CoM heterodisulfide,ferredoxin reductase

for references in articles please use BRENDA:EC1.8.98.5
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The expected taxonomic range for this enzyme is: Methanothermobacter marburgensis

EC NUMBER
COMMENTARY hide
1.8.98.5
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RECOMMENDED NAME
GeneOntology No.
H2:CoB-CoM heterodisulfide,ferredoxin reductase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2 reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + 2 H+ = 2 H2 + 2 oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
coenzyme B/coenzyme M regeneration IV (H2-dependent)
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Methane metabolism
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Metabolic pathways
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Microbial metabolism in diverse environments
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SYSTEMATIC NAME
IUBMB Comments
CoB,CoM,ferredoxin:H2 oxidoreductase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Methanobacterium thermoautotrophicus
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Manually annotated by BRENDA team
Methanobacterium thermoautotrophicus DSM 2133
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
coenzyme B-coenzyme M disulfide + ferredoxin + 2 H2
coenzyme B + coenzyme M + reduced ferredoxin2- + 2 H+
show the reaction diagram
coenzyme B-coenzyme M disulfide + metronidazole + 2 H2
coenzyme B + coenzyme M + reduced metronidazole2- + 2 H+
show the reaction diagram
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
show the reaction diagram
H2 + oxidized methyl viologen + CoM-S-S-CoB
reduced methyl viologen + CoB + CoM + H+
show the reaction diagram
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
show the reaction diagram
reduced metronidazole + CoB + CoM + H+
H2 + oxidized metronidazole + CoM-S-S-CoB
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
show the reaction diagram
reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
H2 + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
Methanobacterium thermoautotrophicus
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the enzyme contains approximately 0.9 mol nickel
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
Methanobacterium thermoautotrophicus
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the enzyme contains approximately 26 mol non-heme iron
Ni2+
Methanobacterium thermoautotrophicus
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the enzyme contains approximately 0.6 mol nickel
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
coenzyme F430
Methanobacterium thermoautotrophicus
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potassium phosphate
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110
HdrABC complex with 100% H2 in the gas phase, pH 7.6, 60°C; HdrABC complex with 100% H2 in the gas phase, pH 7.6, 60°C; HdrABC complex with 100% H2 in the gas phase, pH 7.6, 60°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
250000
Methanobacterium thermoautotrophicus
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterohexamer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose and Q-Sepharose column chromatography, and Superdex 200 gel filtration
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DEAE-Sepharose column chromatography, phenyl-Sepharose column chromatography, and hydroxyapatite column chromatography
Methanobacterium thermoautotrophicus
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DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration
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