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Information on EC 1.8.4.B3 - thioredoxin-independent methionine sulfoxide reductases B

for references in articles please use BRENDA:EC1.8.4.B3

preliminary BRENDA-supplied EC number

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EC Tree

1 Oxidoreductases

1.8 Acting on a sulfur group of donors

1.8.4 With a disulfide as acceptor

1.8.4.B3 thioredoxin-independent methionine sulfoxide reductases B

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The enzyme appears in viruses and cellular organisms

Reaction Schemes

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peptide-L-methionine-(R)-S-sulfoxide

Synonyms

hMsrB2, hMsrB3, NtMsrB1, thioredoxin-independent methionine sulfoxide reductases B, show all synonyms

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

hMsrB2

hMsrB3

NtMsrB1

thioredoxin-independent methionine sulfoxide reductases B

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

peptide-L-methionine-(R)-S-sulfoxide + thionein = peptide-L-methionine + thionein disulfide + H2O

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein

dabsyl-L-methionine + bovine liver thionein + H2O

Nicotiana tabacum

A7U630

NtMsrB1

684867

dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol

dabsyl-L-methionine + dithiothreitol disulfide + H2O

Nicotiana tabacum

A7U630

NtMsrB1

684867

dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32

dabsyl-L-methionine + ?

Nicotiana tabacum

A7U630

NtMsrB1

684867

dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine

dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O

Nicotiana tabacum

A7U630

NtMsrB1

684867

L-methionine (R)-S-sulfoxide + dithiothreitol

L-methionine + dithiothreitol disulfide + H2O

Homo sapiens

Q8IXL7, Q9Y3D2

670705

L-methionine (R)-S-sulfoxide + thionein

L-methionine + thionein disulfide + H2O

Homo sapiens

Q8IXL7, Q9Y3D2

670705

L-methionine (R)-S-sulfoxide + thioredoxin

L-methionine + thioredoxin disulfide

Homo sapiens

Q8IXL7, Q9Y3D2

reduced thioredoxin is not an efficient reducing agent for hMsrB3. Less than 5% of the activity with dithiothreitol

670705

L-methionine (R)-S-sulfoxide + thioredoxin

L-methionine + thioredoxin disulfide + H2O

Homo sapiens

Q8IXL7, Q9Y3D2

reduced thioredoxin is not an efficient reducing agent for hMsrB2. Less than 5% of the activity with dithiothreitol

670705

L-methionine (R)-sulfoxide + dithiothreitol

L-methionine + dithiothreitol disulfide

Homo sapiens

Q8IXL7, Q9Y3D2

670705

peptide-L-methionine-(R)-S-sulfoxide + thionein

peptide-L-methionine + thionein disulfide + H2O

Nicotiana tabacum

A7U630

684867

additional information

4 entries

additional information

Homo sapiens

Q8IXL7

zinc-containing metallothionein in the presence of EDTA can serve as a reducing agent. Thioredoxin can reduce oxidized thionein

670705

additional information

Homo sapiens

Q9Y3D2

zinc-containing metallothionein in the presence of EDTA can serve as a reducing agent. Thioredoxin can reduce oxidized thionein

670705

additional information

Nicotiana tabacum

A7U630

NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction

684867

additional information

Nicotiana tabacum

684867

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

peptide-L-methionine-(R)-S-sulfoxide + thionein

peptide-L-methionine + thionein disulfide + H2O

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Homo sapiens

670705

Q8IXL7, Q9Y3D2

SwissProt

brenda

Nicotiana tabacum

precursor, fragment

684867

A7U630

SwissProt

brenda

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

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LOCALIZATION

ORGANISM

UNIPROT

COMMENTARY

GeneOntology No.

LITERATURE

SOURCE

brenda

Go to AA Sequence and Transmembrane Helices Search

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

Sequence

A7U630 pBLAST

A7U630_TOBAC

Nicotiana tabacum

195

21413

TrEMBL

Show Sequence

Q8IXL7 pBLAST

MSRB3_HUMAN

Homo sapiens

192

20702

Swiss-Prot

Show Sequence

Q9Y3D2 pBLAST

MSRB2_HUMAN

Homo sapiens

182

19536

Swiss-Prot

Show Sequence

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

15200

Nicotiana tabacum

A7U630

x * 15200, calculated from sequence

684867

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Nicotiana tabacum

A7U630

x * 15200, calculated from sequence

684867

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Go to Cloned (commentary) Search

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli BL21

Nicotiana tabacum

A7U630

684867

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

670705

Sagher, D.; Brunell, D.; Hejtmancik, J.F.; Kantorow, M.; Brot, N.; Weissbach, H.

Thionein can serve as a reducing agent for the methionine sulfoxide reductases

Proc. Natl. Acad. Sci. USA

103

8656-8661

2006

Homo sapiens, Homo sapiens (Q8IXL7), Homo sapiens (Q9Y3D2)

16735467

brenda

684867

Ding, D.; Sagher, D.; Laugier, E.; Rey, P.; Weissbach, H.; Zhang, X.H.

Studies on the reducing systems for plant and animal thioredoxin-independent methionine sulfoxide reductases B

Biochem. Biophys. Res. Commun.

361

629-633

2007

Nicotiana tabacum (A7U630), Nicotiana tabacum

17673175

brenda

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EXTERNAL LINKS (specific for EC-Number 1.8.4.B3)

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

IUBMB Enzyme Nomenclature

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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