BRENDA - Enzyme Database show
show all sequences of 1.8.4.B3

Studies on the reducing systems for plant and animal thioredoxin-independent methionine sulfoxide reductases B

Ding, D.; Sagher, D.; Laugier, E.; Rey, P.; Weissbach, H.; Zhang, X.H.; Biochem. Biophys. Res. Commun. 361, 629-633 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli BL21
Nicotiana tabacum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Nicotiana tabacum
9507
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15200
-
x * 15200, calculated from sequence
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
peptide-L-methionine-(R)-S-sulfoxide + thionein
Nicotiana tabacum
-
peptide-L-methionine + thionein disulfide + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
A7U630
precursor, fragment
-
Purification (Commentary)
Commentary
Organism
-
Nicotiana tabacum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Nicotiana tabacum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + bovine liver thionein + H2O
-
-
-
?
dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
-
-
?
dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + ?
-
-
-
?
dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O
-
-
-
?
additional information
NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction
684867
Nicotiana tabacum
?
-
-
-
-
peptide-L-methionine-(R)-S-sulfoxide + thionein
-
684867
Nicotiana tabacum
peptide-L-methionine + thionein disulfide + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 15200, calculated from sequence
Nicotiana tabacum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli BL21
Nicotiana tabacum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
chloroplast
-
Nicotiana tabacum
9507
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
15200
-
x * 15200, calculated from sequence
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
peptide-L-methionine-(R)-S-sulfoxide + thionein
Nicotiana tabacum
-
peptide-L-methionine + thionein disulfide + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nicotiana tabacum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Nicotiana tabacum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + bovine liver thionein + H2O
-
-
-
?
dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + dithiothreitol disulfide + H2O
-
-
-
?
dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + ?
-
-
-
?
dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O
-
-
-
?
additional information
NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction
684867
Nicotiana tabacum
?
-
-
-
-
peptide-L-methionine-(R)-S-sulfoxide + thionein
-
684867
Nicotiana tabacum
peptide-L-methionine + thionein disulfide + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 15200, calculated from sequence
Nicotiana tabacum
Other publictions for EC 1.8.4.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
684867
Ding
Studies on the reducing system ...
Nicotiana tabacum
Biochem. Biophys. Res. Commun.
361
629-633
2007
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7
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6
1
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1
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1
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1
1
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1
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1
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6
1
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670705
Sagher
Thionein can serve as a reduci ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
8656-8661
2006
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6
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8
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8
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