Data extracted from this reference:
Cloned(Commentary)
expression in Escherichia coli BL21
Nicotiana tabacum
Localization
chloroplast
Nicotiana tabacum
9507
Molecular Weight [Da]
15200
x * 15200, calculated from sequence
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
peptide-L-methionine-(R)-S-sulfoxide + thionein
Nicotiana tabacum
peptide-L-methionine + thionein disulfide + H2O
?
Organism
Nicotiana tabacum
A7U630
precursor, fragment
Purification (Commentary)
Source Tissue
Substrates and Products (Substrate)
dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + bovine liver thionein + H2O
?
dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + dithiothreitol disulfide + H2O
?
dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + ?
?
dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O
?
additional information
NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction
684867
Nicotiana tabacum
?
peptide-L-methionine-(R)-S-sulfoxide + thionein
684867
Nicotiana tabacum
peptide-L-methionine + thionein disulfide + H2O
?
Subunits
?
x * 15200, calculated from sequence
Nicotiana tabacum
Synonyms
NtMsrB1
Nicotiana tabacum
thioredoxin-independent methionine sulfoxide reductases B
Nicotiana tabacum
Cloned(Commentary) (protein specific)
expression in Escherichia coli BL21
Nicotiana tabacum
Localization (protein specific)
chloroplast
Nicotiana tabacum
9507
Molecular Weight [Da] (protein specific)
15200
x * 15200, calculated from sequence
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
peptide-L-methionine-(R)-S-sulfoxide + thionein
Nicotiana tabacum
peptide-L-methionine + thionein disulfide + H2O
?
Purification (Commentary) (protein specific)
Source Tissue (protein specific)
Substrates and Products (Substrate) (protein specific)
dabsyl-L-methionine (R)-S-sulfoxide + bovine liver thionein
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + bovine liver thionein + H2O
?
dabsyl-L-methionine (R)-S-sulfoxide + dithiothreitol
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + dithiothreitol disulfide + H2O
?
dabsyl-L-methionine (R)-S-sulfoxide + plant Trx-like protein CDSP32
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + ?
?
dabsyl-L-methionine (R)-S-sulfoxide + selenocysteamine
NtMsrB1
684867
Nicotiana tabacum
dabsyl-L-methionine + 2,2'-disulfanediyldiethanamine + H2O
?
additional information
NtMsrB1 shows very little activity towards L-methionine (R)-sulfoxide. Escherichia coli thioredoxin can not serve as a reducing agent for NtMsrB1. The Trx-independent MsrB enzyme lacks an additional cysteine (resolving cysteine) that is capable of forming a disulfide bond on the enzyme during the catalytic reaction
684867
Nicotiana tabacum
?
peptide-L-methionine-(R)-S-sulfoxide + thionein
684867
Nicotiana tabacum
peptide-L-methionine + thionein disulfide + H2O
?
Subunits (protein specific)
?
x * 15200, calculated from sequence
Nicotiana tabacum
Other publictions for EC 1.8.4.B3
684867
Ding
Studies on the reducing system ...
Nicotiana tabacum
Biochem. Biophys. Res. Commun.
361
629-633
2007
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1
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1
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1
1
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7
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1
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1
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6
1
2
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1
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1
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1
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1
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1
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6
1
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670705
Sagher
Thionein can serve as a reduci ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
103
8656-8661
2006
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8
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