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4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
4-toluenesulfonate + NADH + O2
?
additional information
?
-
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: -
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: -
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: -
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: -
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: -
Products: -
?
4-toluenesulfonate + NADH + O2
?
-
Substrates: the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
Products: -
?
4-toluenesulfonate + NADH + O2
?
-
Substrates: this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC)
Products: -
?
4-toluenesulfonate + NADH + O2
?
-
Substrates: this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC)
Products: -
?
4-toluenesulfonate + NADH + O2
?
-
Substrates: this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC)
Products: -
?
4-toluenesulfonate + NADH + O2
?
-
Substrates: the oxygenase component was expressed at high levels, no expression of a specific reductase activity linked to synthesis of the oxygenase could be detected
Products: -
?
4-toluenesulfonate + NADH + O2
?
-
Substrates: this strain requires the reductase (TsaB) of toluenesulfonate methyl monooxygenase in the incompletely expressed sulfobenzoate dioxygenase system (PsbAC)
Products: -
?
additional information
?
-
-
Substrates: poor substrates: 2-sulfobenzoate, 3-sulfobenzoate, benzenesulfonate, 4-methylbenzenesulfonate, 4-hydroxybenzenesulfonate, 4-aminobenzenesulfonate, 4-nitrobenzenesulfonate, 4-chloro-benzenesulfonate, 4-sulfophenylacetate, 4-sulfophenylpropionate, 4-sulfophenylbutyrate, no substrates are: benzoate, 4-methylbenzoate, 4-hydroxybenzoate, 4-aminobenzoate, 4-nitrobenzoate, 4-chlorobenzoate
Products: -
?
additional information
?
-
-
Substrates: poor substrates: 2-sulfobenzoate, 3-sulfobenzoate, benzenesulfonate, 4-methylbenzenesulfonate, 4-hydroxybenzenesulfonate, 4-aminobenzenesulfonate, 4-nitrobenzenesulfonate, 4-chloro-benzenesulfonate, 4-sulfophenylacetate, 4-sulfophenylpropionate, 4-sulfophenylbutyrate, no substrates are: benzoate, 4-methylbenzoate, 4-hydroxybenzoate, 4-aminobenzoate, 4-nitrobenzoate, 4-chlorobenzoate
Products: -
?
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4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity
Products: -
?
4-sulfobenzoate + NADH + O2
3,4-dihydroxybenzoate + sulfite + NAD+
-
Substrates: reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity
Products: -
?
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105000
-
oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Sephadex G-2000 gel filtration
36000
-
1 * 36000, reductase B, SDS-PAGE
39000
-
reductase B, contains 1 mol of FMN and about 2 mol each of iron and inorganic sulfur per mol, Superose gel filtration
47000
-
reductase B, Sephadex G-2000 gel filtration
85000
-
oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Superose gel filtration
additional information
-
simple multi-component dioxygenase of two, perhaps three components: reductase B coupled to dimeric oxygenase, reductase C exhibits only 15% of total activity
50000
-
dioxygenase A, SDS-PAGE
50000
-
2 * 50000, oxygenase A, SDS-PAGE
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Junker, F.; Saller, E.; Schlfli Oppenberg, H.R.; Kroneck, P.M.H.; Leisinger, T.; Cook, A.M.
Degradative pathways for p-toluenecarboxylate and p-toluenesulfonate and their multicomponent oxygenases in Comamonas testosteroni strains PSB-4 and T-2
Microbiology
142
2419-2427
1996
Comamonas testosteroni, Comamonas testosteroni PSB-4, Comamonas testosteroni T-2
brenda
Schlfli Oppenberg, H.R.; Chen, G.; Leisinger, T.; Cook, A.M.
Regulation of the degradative pathways from 4-toluenesulphonate and 4-toluenecarboxylate to protocatechuate in Comamonas testosteroni T-2
Microbiology
141
1891-1899
1995
Comamonas testosteroni, Comamonas testosteroni T-2
brenda
Locher, H.H.; Leisinger, T.; Cook, A.M.
4-Sulphobenzoate 3,4-dioxygenase. Purification and properties of a desulphonative two-component enzyme system from Comamonas testosteroni T-2
Biochem. J.
274
833-842
1991
Comamonas testosteroni, Comamonas testosteroni T-2
brenda