Ligand sulfite

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Basic Ligand Information

Molecular Structure
Picture of sulfite (click for magnification)
Molecular Formula
BRENDA Name
InChIKey
H2O3S
sulfite
LSNNMFCWUKXFEE-UHFFFAOYSA-L
Synonyms:
(HSO3)-, bisulfite, HSO3-, hydrogen sulfite, hydrogensulfite, SO32-, sulfurous acid, Sulphite

Roles as Enzyme Ligand

In Vivo Substrate in Enzyme-catalyzed Reactions (39 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
sulfite + reduced coenzyme F420 = hydrogen sulfide + oxidized coenzyme F420
show the reaction diagram
-
2-hydroxybiphenyl + sulfite = 2'-hydroxybiphenyl-2-sulfinate + H2O
show the reaction diagram
-
L-Cys + sulfite = ?
show the reaction diagram
-

In Vivo Product in Enzyme-catalyzed Reactions (46 results)

EC NUMBER
PROVEN IN VIVO REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
taurine + 2-oxoglutarate + O2 = sulfite + aminoacetaldehyde + succinate + CO2
show the reaction diagram
-
-
4-sulfobenzoate + NADH + O2 = 3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
-
thiosulfate + 2 ferrocytochrome c3 = sulfite + hydrogen sulfide + 2 ferricytochrome c3
show the reaction diagram
-
-
hydrogen sulfide + oxidized ferredoxin + H2O = sulfite + reduced ferredoxin + H+
show the reaction diagram
-
-
2-sulfoacetaldehyde + phosphate = acetyl phosphate + sulfite
show the reaction diagram
-
-
thiosulfate + cyanide = sulfite + thiocyanate
show the reaction diagram
-
-
thiosulfate + glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
-
thiosulfate + 2 dithioerythritol = sulfite + dithioerythritol disulfide + sulfide
show the reaction diagram
-
-
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite
show the reaction diagram
-
-
3-sulfolactate = pyruvate + bisulfite
show the reaction diagram
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-
L-cysteate + H2O = pyruvate + bisulfite + NH3
show the reaction diagram
-
-

Substrate in Enzyme-catalyzed Reactions (135 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
sulfite + hydrogen sulfide + menaquinone = thiosulfate + menaquinol
show the reaction diagram
-
sulfite + ferricyanide = sulfate + ferrocyanide
show the reaction diagram
-
L-phosphoserine + sulfite = L-cysteate + phosphate
show the reaction diagram
-
thiosulfate + sulfite = sulfite + thiosulfate
show the reaction diagram
-
2-hydroxybiphenyl + sulfite = 2'-hydroxybiphenyl-2-sulfinate + H2O
show the reaction diagram
-

Product in Enzyme-catalyzed Reactions (142 results)

EC NUMBER
REACTION
REACTION DIAGRAM
LITERATURE
ENZYME 3D STRUCTURE
3-sulfocatechol + O2 + H2O = (2E,4Z)-2-hydroxymuconate + bisulfite + H+
show the reaction diagram
-
-
4-sulfobenzoate + NADH + O2 = 3,4-dihydroxybenzoate + sulfite + NAD+
show the reaction diagram
-
thiosulfate + menaquinol = sulfite + hydrogen sulfide + menaquinone
show the reaction diagram
-
-
2-sulfoacetaldehyde + phosphate = acetyl phosphate + sulfite
show the reaction diagram
-
-
4-aminobutyrate + 2-oxoglutarate = L-glutamate + acetaldehyde + sulfite
show the reaction diagram
-
-
thiosulfate + glutathione = sulfite + glutathione disulfide + sulfide
show the reaction diagram
-
L-cysteine sulfinic acid = L-alanine + sulfite
show the reaction diagram
-
4-sulfomuconolactone + H2O = maleylacetate + sulfite
show the reaction diagram
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-
UDP-alpha-D-sulfoquinovopyranose + H2O = UDP-alpha-D-glucose + sulfite
show the reaction diagram
-
-

Activator in Enzyme-catalyzed Reactions (23 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
the addition of sulfite (2 mM) results in the activation of the enzyme at a magnitude of 1.6fold
-
13.6fold increase in the rate of H2S oxidation in the presence of 0.6 mM sulfite
-
activation, together with SDS or guanidine hydrochloride
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activating effect at high thiosulfate concentrations in p-dimethylaminoazobenzene-treated mice
-
1.0 mM, relative activity 105%
-
stimulates
-
stimulates activity
-

Inhibitor in Enzyme-catalyzed Reactions (146 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
competitive
sulfite build up can potentially inhibit PDO activity
-
irreversible inactivation is Cu2+-dependent
-
37.5% inhibtion at 15 mM after 6 h incubation of cells
-
inhibition of activity with NADP+ but not with methyl viologen
-
non-competitive inhibitor with respect to both NADPH and 3-phosphoglycerate
-
in presence of substrate
-
uncompetitive
-
1 mM, 30% inhibition
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preincubation of SoxF with 1 mM sodium sulfite for 20 min inactivates SoxF (9.0% residual sulfide dehydrogenase activity)
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0.1 mM, complete inhibition
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above 1 mM, product inhibition
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competitive to sulfide
-
1 mM, 10-25% inhibition
-
enzymatic activity: 1.6%
-
0.2 mM 97% inhibition
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increasing concentrations beyond 0.1 mM
-
reversible
-
inhibitory effect increases rapidly with time to a constant level, not competitve with substrate
-
noncompetitive, inhibition by a time-dependent mechanism, addition of succinic semialdehyde protects against sulfite inhibition
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inhibitory at 20 mM
-
substrate inhibition above 3 mM
-
noncompetitive
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Metals and Ions (7 results)

EC NUMBER
COMMENTARY
LITERATURE
ENZYME 3D STRUCTURE
binds at the active site as arsenite analogue
-
activates 1.6fold at 2 mM, higher concentration of sulfite ions results in inhibition of the enzyme, and almost complete inhibition at 10 mM sulfite
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stimulates
-
activates
-
reduction is essential for catalytic activity
-
K2SO3, absolute requirement in the reactivation of the inactive enzyme complex with hydroxocobalamin
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3D Structure of Enzyme-Ligand-Complex (PDB) (54 results)

Enzyme Kinetic Parameters

kcat Value (Turnover Number) (221 results)

EC NUMBER
TURNOVER NUMBER [1/S]
TURNOVER NUMBER MAXIMUM [1/S]
COMMENTARY
LITERATURE
0.31
-
pH 7.6, 22°C

KM Value (295 results)

EC NUMBER
KM VALUE [MM]
KM VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
60
-
in 0.1 M K+-phosphate buffer, at pH 7.0 and 30°C
0.012
-
pH 7.0, temperature not specified in the publication
0.01
-
-

Ki Value (23 results)

EC NUMBER
KI VALUE [MM]
KI VALUE MAXIMUM [MM]
COMMENTARY
LITERATURE
0.0014
-
-
0.7
-
-
60
-
-
0.3
-
sulfite resembles the sulfonate part of thiosulfate and might, therefore, block the active site of the enzyme
0.005
-
; pH 6.0, 80°C

IC50 Value (1 result)

EC NUMBER
IC50 VALUE
IC50 VALUE MAXIMUM
COMMENTARY
LITERATURE
0.59
-
pH 7.5, 20°C, inhibition of CO2 hydration activity

References & Links