General Stability | Organism |
---|---|
ammonium sulfate precipitation leads to excessive loss of activity | Comamonas testosteroni |
chloride-buffers result in diminished yields during purification | Comamonas testosteroni |
glycerol, 30% v/v, stabilizes | Comamonas testosteroni |
repeated freezing and thawing inactivates | Comamonas testosteroni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | 0.032 | 4-Sulfobenzoate | - |
Comamonas testosteroni |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Comamonas testosteroni | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | requirement, iron-sulfur protein, 1 mol (2Fe-2S) per mol reductase B, one 2Fe-2S center (Rieske-type) per oxygenase A subunit, atomic absorption spectroscopy | Comamonas testosteroni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
simple multi-component dioxygenase of two, perhaps three components: reductase B coupled to dimeric oxygenase, reductase C exhibits only 15% of total activity | Comamonas testosteroni |
36000 | - |
1 * 36000, reductase B, SDS-PAGE | Comamonas testosteroni |
39000 | - |
reductase B, contains 1 mol of FMN and about 2 mol each of iron and inorganic sulfur per mol, Superose gel filtration | Comamonas testosteroni |
47000 | - |
reductase B, Sephadex G-2000 gel filtration | Comamonas testosteroni |
50000 | - |
2 * 50000, oxygenase A, SDS-PAGE | Comamonas testosteroni |
85000 | - |
oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Superose gel filtration | Comamonas testosteroni |
105000 | - |
oxygenase A, native dioxygenase: the enzyme is presumed to be homodimeric, Sephadex G-2000 gel filtration | Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-sulfobenzoate + NADH + O2 | Comamonas testosteroni | reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity | 3,4-dihydroxybenzoate + sulfite + NAD+ | - |
? | |
4-sulfobenzoate + NADH + O2 | Comamonas testosteroni T-2 | reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity | 3,4-dihydroxybenzoate + sulfite + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
T-2 | - |
Comamonas testosteroni T-2 | - |
T-2 | - |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
reductase B | Comamonas testosteroni |
0.8 | - |
oxygenase A | Comamonas testosteroni |
84 | - |
reductase B, dichlorophenolindophenol | Comamonas testosteroni |
138 | - |
reductase B, cytochrome c | Comamonas testosteroni |
318 | - |
reductase B, ferricyanide | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-sulfobenzoate + NADH + O2 | - |
Comamonas testosteroni | 3,4-dihydroxybenzoate + sulfite + NAD+ | - |
? | |
4-sulfobenzoate + NADH + O2 | reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity | Comamonas testosteroni | 3,4-dihydroxybenzoate + sulfite + NAD+ | - |
? | |
4-sulfobenzoate + NADH + O2 | - |
Comamonas testosteroni T-2 | 3,4-dihydroxybenzoate + sulfite + NAD+ | - |
? | |
4-sulfobenzoate + NADH + O2 | reaction in toluene-p-sulfonate degradation pathway, simple multi-component dioxygenase of 2, perhaps 3 components: reductase B coupled to dimeric oxygenase, the third component, reductase C, represents only 15% of total activity | Comamonas testosteroni T-2 | 3,4-dihydroxybenzoate + sulfite + NAD+ | - |
? | |
additional information | poor substrates: 2-sulfobenzoate, 3-sulfobenzoate, benzenesulfonate, 4-methylbenzenesulfonate, 4-hydroxybenzenesulfonate, 4-aminobenzenesulfonate, 4-nitrobenzenesulfonate, 4-chloro-benzenesulfonate, 4-sulfophenylacetate, 4-sulfophenylpropionate, 4-sulfophenylbutyrate, no substrates are: benzoate, 4-methylbenzoate, 4-hydroxybenzoate, 4-aminobenzoate, 4-nitrobenzoate, 4-chlorobenzoate | Comamonas testosteroni | ? | - |
? | |
additional information | poor substrates: 2-sulfobenzoate, 3-sulfobenzoate, benzenesulfonate, 4-methylbenzenesulfonate, 4-hydroxybenzenesulfonate, 4-aminobenzenesulfonate, 4-nitrobenzenesulfonate, 4-chloro-benzenesulfonate, 4-sulfophenylacetate, 4-sulfophenylpropionate, 4-sulfophenylbutyrate, no substrates are: benzoate, 4-methylbenzoate, 4-hydroxybenzoate, 4-aminobenzoate, 4-nitrobenzoate, 4-chlorobenzoate | Comamonas testosteroni T-2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, oxygenase A, SDS-PAGE | Comamonas testosteroni |
monomer | 1 * 36000, reductase B, SDS-PAGE | Comamonas testosteroni |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at, reductase B | Comamonas testosteroni |
30 | - |
assay at | Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Comamonas testosteroni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | flavoprotein, 1 mol FMN/mol reductase B | Comamonas testosteroni | |
additional information | no independent ferredoxin | Comamonas testosteroni | |
NADH | - |
Comamonas testosteroni |